Plk0.1 trc cloning vector

The pLK0.1 TRC cloning vector (17 (link)), the pLKO.1-TRC vector (17 (link)), and the scramble shRNA vector in pLKO.1 (18 (link)) were obtained from Addgene (Watertown, Mass). The pMD2.G vector [expressing VSV-G envelop; Addgene plasmid # 12259; http://n2t.net/addgene:12259;RRID:Addgene_12259), and psPAX2 (a lentiviral packaging vector; Addgene plasmid # 12260; http://n2t.net/addgene:12260;RRID:Addgene_12260)] were gifts from Didier Trono.

We previously described the BHRF1-HA expression vector [19 (link)], which was constructed from a pcDNA3.1 expression vector (EV) purchased from Invitrogen (V79020). The mCherry-α-tubulin WT-K40K control vector was kindly provided by Dr. R. Y. Tsien (Howard Hughes Medical Center, University of San Diego, USA) [59 (link)] and the mutated vector (mCherry-α-tubulin K40A) was a kind gift from Dr. F. Saudou (Institut Curie, Orsay, France) [13 (link)]. The GFP-ATAT1 expression plasmid (27099) and the pLK0.1-TRC cloning vector (10878) were purchased from Addgene. The plasmids Flag-ΔRIG-I (2xCARD) and pIFN-β-Luc were previously described [27 (link),60 (link)] and kindly provided by Dr. C. Lagaudrière (I2BC, Gif-sur-Yvette, France). The plasmid pRL-TK was purchased from Promega Corporation (E2241). The p50-dynamitin construct was a gift from Dr. S. Etienne-Manneville (Institut Pasteur, Paris, France) [61 (link)]. ZEBRA and Rta expression plasmids were kindly provided by Henri Gruffat (CIRI, Lyon, France). The AMBRA1-ActA expression plasmid was previously described [30 (link)] and a gift from Flavie Strappazzon. The plasmids psPAX2 (Addgene, 12260) and pMD2.G (Addgene, 12259) come from Didier Trono lab (EPFL, Lausanne, Switzerland).