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Anti primpol

Manufactured by Abcam
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Anti-PRIMPOL is a primary antibody that targets the PRIMPOL protein. PRIMPOL is a DNA-dependent primase and a DNA-dependent polymerase involved in DNA replication and repair processes. The antibody can be used to detect and study the PRIMPOL protein in various applications.

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Lab products found in correlation

Anti rpa2, by Abcam (2 mentions) Anti flag tag, by Cell Signaling Technology (2 mentions) Anti rad51, by Abcam (2 mentions) Anti β tubulin, by Immunoway (2 mentions) Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Alexa fluor 555 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Alexa fluor 555 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Chemiluminescence system, by Merck Group (1 mentions) Hrp conjugated secondary antibodies, by Immunoway (1 mentions) Pvdf membrane, by Merck Group (1 mentions)

2 protocols using anti primpol

1

Immunofluorescence Assay for DNA Repair Proteins

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The antibodies used are as follows: anti-FLAG-tag (Cell Signaling Technology, Danvers, USA), anti-RPA2 (Abcam, Cambridge, UK), anti-β-tubulin (Immunoway, Plano, USA), anti-PRIMPOL (Abcam), anti-RAD51 (Abcam), goat-anti-mouse Alexa Fluor 488 (Thermo Scientific, Waltham, USA), goat-anti-mouse Alexa Fluor 555 (Thermo Scientific), goat-anti-rabbit Alexa Fluor 488 (Thermo Scientific), and goat-anti-rabbit Alexa Fluor 555 (Thermo Scientific).
Li T., Tang L., Kou H, & Wang F. (2022). PRIMPOL competes with RAD51 to resolve G-quadruplex-induced replication stress via its interaction with RPA: PRIMPOL resolves G4-induced replication stress. Acta Biochimica et Biophysica Sinica, 55(3), 498-507.
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2

Western Blot Analysis of Cellular Proteins

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Cell extracts were harvested and separated by SDS-PAGE, and then transferred to PVDF membranes (Millipore, Billerica, USA). The membranes were blocked with 5% non-fat milk for 1 h at room temperature, washed, and incubated with specific antibodies overnight at 4°C. The membranes were washed again and incubated with the corresponding HRP-conjugated secondary antibodies (Immunoway). Finally, protein bands were detected using a chemiluminescence system (Millipore). The primary antibodies used are as follows: anti-FLAG-tag (Cell Signaling Technology), anti-RPA2 (Abcam), anti-β-tubulin (Immunoway), anti-PRIMPOL (Abcam), and anti-RAD51 (Abcam).
Li T., Tang L., Kou H, & Wang F. (2022). PRIMPOL competes with RAD51 to resolve G-quadruplex-induced replication stress via its interaction with RPA: PRIMPOL resolves G4-induced replication stress. Acta Biochimica et Biophysica Sinica, 55(3), 498-507.
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