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1

Enzymatic Digestion of Proteins

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Water used in all the experiments was obtained from a reverse osmosis water purification system. Trypsin (1,000–2,000 U/mg) and α-amylase (≥5,000 U/g) from the porcine pancreas were purchased from Sigma-Aldrich, Switzerland. Pepsin (≥250 U/mg) from porcine gastric mucosa, serine, and artificial saliva (pH = 6.8) were obtained from Solarbio, Beijing, CN. Pancreatin (≥4,000 U/g), o-phthalaldehyde, and bovine serum albumin were bought from Yuanye, Shanghai, CN. Ethanol and petroleum ether were purchased from Aladdin, Shanghai, CN. All the chemical reagents used were of analytical grade.
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2

Ziziphus jujuba Leaf Metabolite Profiling

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Leaves from the Ziziphus jujuba Mill. cv. Dongzao were used, and the picking standard is two leaves and one bud on the fruit-bearing shoot (Figure 1C). The leaves were collected in June 2022 in Baoding (115° 47’E; 38° 87’N) (Hebei Province, China). Liquid chromatography grade solvents methanol and acetonitrile were purchased from Fisher Chemical, 2-propanol from Merck, formal acid from CNW, and 2-Chloro-L-phenylAlanine (≥98%) from Adamas-beta. Alanine, arginine, asparagine, aspartic acid, cystine, glutamine, glutamic acid, glycine, histidine, isoleucine, L-cysteine, leucine, L-hydroxyproline, L-tryptophan, lysine, methionine, phenylAlanine, proline, serine, threonine, tyrosine, valine, and 1,3-dichlorobenzene were all analytically pure and purchased from Beijing Solarbio Science&Technology Co., Ltd.
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3

Bioactive Compound Analytical Methods

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1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Ferric-tripyridyltriazine (Fe3+-TPTZ) is purchased from Beyotime Biotechnology (Shanghai, China). Chromatographic grade analytical standards asparaginic acid, glutamic acid, asparagine, serine, glutamine, histidine, glycine, threonine, citrulline, arginine, alanine, tyrosine, cystine, valine, methionine, tryptophan, phenylalanine, isoleucine, leucine, lysine, hydroxyproline, proline, 5′-CMP, 5′-UMP, 5′-GMP, 5′-IMP, 5′-XMP, 5′-AMP, trehalose, mannitol, quininic acid, l-malic acid and fumaric acid purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). α-Amylase, α-glucosidase, 4-nitrophenyl α-d-glucopyranoside (PNPG), and soluble starch were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). All other reagents were analytical grade and obtained from Chengdu Kelong Chemical Factory (Chengdu, China).
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4

Synthesis and Characterization of Organic and Inorganic Compounds

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ATP, p-toluidine, 5-ASA, 3-aminosalicylic acid (3-ASA), 4-aminosalicylic acid (4-ASA) were obtained in Shanghai Perfemiker Material Tech Co., Ltd (Shanghai, China, http://www.canspecsci.com). Fe(NO3)3, CuCl2, HgCl2, NiCl2, CdCl2, CrCl3, ZnCl2, CaCl2, Pb(NO3)2, AgNO3, Co(NO3)2·6H2O, Mn(Ac)2, MgCl2, BaCl2 and Bi(NO3)3·5H2O were purchased from Fluka (Buchs, Switzerland, http://www.fluka.org/fluka.php). p-Nitrochlorobenzene, o-nitrobenzaldehyde, p-nitrotoluene, salicylic acid, benzidine, p-chlorophenol, acetanilide, o-aminophenol, alanine, leucine, phenylalanine, glutamic acid, asparagine, arginine, tyrosine, histidine, serine, glycine, valine, tryptophan, lysine, glutathione, cysteine, melamine and ethanol were purchased in Beijing Solarbio Reagent Co., Ltd (Beijing, China, http://www.solarbio.com). Unless otherwise specified, all chemicals from commercial sources are analytically pure and can be used directly without further purification. All solutions were prepared and diluted with ultrapure water from the MillI-Q water purification system.
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