We used, in this study, the following reagents: Sulfuric acid (Sigma-Aldrich, St. Louis, MO, USA), nitric acid (Sigma Aldrich), hydrochloric acid, glucose, zinc sulfate, barium hydroxide, potassium acetate (Laboratory chemicals-Lebanon), bovine serum albumin (Sigma-Aldrich CAS 9048-46-8), acetone (Supelco), dichloromethane (Sigma-Aldrich), methanol (Sigma-Aldrich), Folin–Ciocalteu phenol (Sigma-Aldrich CAS 47641), ethanol (Sigma -Aldrich), aluminum chloride (Sigma-Aldrich), 2,2-diphenyl-1-picrylhydrazyl (Sigma-Aldrich), quercetin (Sigma-Aldrich), Dulbecco’s Modified Eagle’s Medium (Sigma-Aldrich CAS D5796), fetal bovine serum (Sigma-Aldrich CAS F9665), phosphate-buffered saline (Sigma-Aldrich, CAS 806552), penicillin/streptomycin (Sigma Aldrich, P4333), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (Sigma-Aldrich CAS M56655), and trypsin (Sigma Aldrich CAS T3924).
Folin ciocalteu phenol
Manufactured by Merck Group
Sourced in United States
The Folin–Ciocalteu phenol is a reagent used for the colorimetric determination of phenolic compounds. It is a mixture of phosphomolybdic and phosphotungstic acids. The reagent undergoes a redox reaction with phenolic compounds, resulting in the formation of a blue-colored complex, which can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (3 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Sulfuric acid, by Merck Group (1 mentions)
Nitric acid, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Dichloromethane, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Aluminum chloride, by Merck Group (1 mentions)
Linoleic acid, by Merck Group (1 mentions)
8 protocols using folin ciocalteu phenol
1
Chemical Reagents for Biological Experiments
2
Antioxidant Matrices Evaluation
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The matrices used were maltodextrin (JR Foods), citrus pectin (JR Foods), commercial skim milk (Svelty Nestle), and whey protein (Nature’s Best). All other chemicals used in the experimental procedures were acetone (Jalmek, NL, Mexico), ethanol (CTR, Monterrey, NL, Mexico), hydrochloric acid (Fermont, Monterrey NL, Mexico), Butanol (Karal, Mexico), ammonium iron (III) sulfate (Meyer, Mexico), formic acid (Jalmek, Mexico), Folin–Ciocalteu phenol (Sigma-Aldrich, USA), sodium carbonate (Jalmek, Mexico), ABTS (Sigma-Aldrich, USA), potassium persulfate (Meyer, Mexico), DPPH (Sigma-Aldrich, Germany), gallic acid (Sigma-Aldrich, USA), methanol (Jalmek, Mexico), acetate buffer (Fermont, Mexico), linoleic acid (Sigma-Aldrich, USA), Tween 20 (Hycel, Mexico), ferrous chloride (Jalmek, Mexico), EDTA (Hycel, Mexico), and sodium hydroxide (Jalmek, Mexico).
3
Wheat Protein Extraction and Characterization
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The two soft winter varieties of wheat, Julius and Ponticus (14.6% and 14.1% total proteins as per Kjedahl method), harvested in 2018 were received from IGV-Institut für Getreideverarbeitung GmbH, Nuthetal OT Bergholz-Rehbrücke, Germany. Chloroform (99.8%), dichloromethane (99.8%), methanol (100%), sodium chloride and ammonium sulfate (99%) were purchased from VWR Chemicals (Leuven, Belgium). Urea was obtained from Merck KGaA (Darmstadt, Germany). Folin & Ciocalteu’ phenol, potassium sodium tartrate tetrahydrate and maltose monohydrate (99%) were acquired from Sigma-Aldrich (Steinheim, Germany). Tris and petroleum ether bp 30–75 °C were obtained from Carl Roth GmbH (Karlsruhe, Germany). 3,5-Dinitrosalicylic acid, 97+% was obtained from Alfa Aesar GmbH (Karlsruhe, Germany). Iodoacetamide (IAA) (PubChem CID: 3727) as well chymotrypsin from bovine pancreas for enzymatic digestion were bought from Sigma Aldrich (St. Louis, MO, USA). Reagents and solutions were all prepared from chemicals by dilution with distilled water.
4
Comprehensive Biochemical Reagents Protocol
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Adenosine triphosphate (ATP), bovine serum albumin (BSA), 1-chloro-2,4-dinitrobenzene (CDNB), dimethylsulfoxide (DMSO), Folin-Ciocalteu phenol, L-glutathione (GSH), α-naphthyl acetate, β-nicotinamide adenine dinucleotide (β-NAD), tetrazotized O-dianisidine (fast blue B salt), trichloroacetic acid (TCA), Tris (hydroxymethyl)aminomethane), triton X-100 and tween 80 were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Other commercially available solvents and chemicals such as ammonium molybdate, copper sulfate, EDTA (ethylenediaminetetraacetic acid), ferrous sulfate, and sodium-potassium tartrate were of analytical grade and purchased from El-Gomhouria For Trading Chemicals And Medical Appliances Co., (Adeb Ishak St, Manshia, Alexandria, Egypt) and used without further purification.
5
Standardized Enzyme and Cell Assays
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Acetylcholinesterase (EC 3.1.1.7), α-amylase (EC 3.2.1.1), α-glucosidase (EC 3.2.1.20), lipase (EC 3.1.1.3), and tyrosinase (EC 1.14.18.1) enzymes, as well as murine RAW 264.7 macrophages and B16 A45 melanoma cell lines, were purchased from Sigma-Aldrich (Darmstadt, Germany). Additionally, all reagents (ATChI, ferric chloride, DTNB, Folin–Ciocalteu phenol, L-glutamine, penicillin, potassium persulfate, streptomycin, and TPTZ), standards (acarbose, galantamine, gallic and kojic acids, L-NAME, melanine, and orlistat), radicals (DPPH and ABTS), and solvents (DMSO and methanol and ethanol of analytical grade) used for chemical analyses were supplied by Sigma-Aldrich (St. Louis, MO, USA).
6
Quantifying Total Phenolic Content
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The total phenolic content was determined using a modified Folin-Ciocalteu procedure [15 (link)]. Briefly, 20 µL of the sample blank solution (80% methanol), gallic acid standards (10–100 µg/mL) and samples were pipetted into their respective wells in a microtiter plate followed by addition of 100 µL of 10% Folin–Ciocalteu phenol (Sigma Aldrich) reagent with gentle mixing by priming using a multichannel pipette. After 5 min, 80 µL of 7% of sodium carbonate was added and primed gently before the plate was covered using an aluminum foil and the reaction left to incubate at room temperature for 90 min. Absorbance readings were obtained at 725 nm in a microtiter plate spectrophotometer reader (Synergy HT, USA). External standard calibration technique was used to quantify the concentration of total phenolic compounds in mg/100 g of the dry sample as Gallic Acid Equivalent (mg GAE).
7
Enzymatic Assay for β-Galactosidase Activity
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β-Galactosidase from Aspergillus oryzae (CAS Number: 9031-11-2), Folin-Ciocalteu phenol, 2,4,6-tripyridyl-s-triazine (TPTZ), 2, 2-diphenyl-2-picrylhydrazyl (DPPH), Rutin and Gallic acid were obtained from Sigma-Aldrich. ο-Nitrophenyl-β-D-Galactopyranoside (CAS Number: 369–07-3) was obtained from ACROS ORGANICS. Other chemicals and solvents were used of analytical grade.
8
Polyphenol Extraction and Quantification
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Trifluoroacetic acid (TFA), anthrone, and Folin-Ciocalteu phenol were purchased from Sigma-Aldrich(Madrid, Spain). Na 2 CO 3 and methanol were from Panreac Química S.A.
(Barcelona, Spain). The standard of gallic acid (GA) was purchased from Sigma-Aldrich. Acetonitrile was of HPLC grade purity (Romyl, Teknokroma, Barcelona, Spain) and acetone was obtained from Sharlau (Barcelo, Spain).
(Barcelona, Spain). The standard of gallic acid (GA) was purchased from Sigma-Aldrich. Acetonitrile was of HPLC grade purity (Romyl, Teknokroma, Barcelona, Spain) and acetone was obtained from Sharlau (Barcelo, Spain).
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