Twist1 2

Manufactured by GeneTex

The Twist1/2 is a laboratory equipment product designed for the detection and analysis of the Twist1 and Twist2 proteins. It provides a reliable and efficient means to measure the expression levels of these proteins, which are involved in various cellular processes. The core function of the Twist1/2 is to facilitate the quantitative assessment of Twist1 and Twist2 expression in biological samples.

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Lab products found in correlation

Gapdh, by Santa Cruz Biotechnology (2 mentions) Py705 stat3, by Cell Signaling Technology (1 mentions) Matrigel, by Corning (1 mentions) Hydrocortisone, by Merck Group (1 mentions) Enhanced chemi luminescence (ecl), by GE Healthcare (1 mentions) Stat3, by BD (1 mentions) Aldh1, by BD (1 mentions) P27kip1, by BD (1 mentions) β actin, by Merck Group (1 mentions) Bca protein assay, by Thermo Fisher Scientific (1 mentions) Vegfa, by Santa Cruz Biotechnology (1 mentions) Py418 src, by Thermo Fisher Scientific (1 mentions) C myc, by Santa Cruz Biotechnology (1 mentions) Snail1, by Cell Signaling Technology (1 mentions) Fibronectin, by Santa Cruz Biotechnology (1 mentions) Gtx108750, by GeneTex (1 mentions) N cadherin 22018 1 ap, by Proteintech (1 mentions) E cadherin 20874 1 ap, by Proteintech (1 mentions) Sc 32233, by Santa Cruz Biotechnology (1 mentions) Ecl substrate kit, by Shanghai Tanon Science & Technology (1 mentions)

Spelling variants of this product

TWIST1 (4 citations) Anti-Twist1/2 (3 citations)

3 protocols using twist1 2

Antibody-based Protein Expression Analysis

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Antibodies to c-Myc (sc-7274), cyclin D1 (sc-753), ErbB-3 (sc-285), Lyn A/B (sc-764), Fyn (sc-16), Src2 (sc-18), VEGF-A (sc-53462), E2A.E12 (sc-349), and ZEB1(sc-10572) (Santa Cruz Biotechnology), p27^Kip1 (BD-Pharmingen 554069), ALDH1 (BD, 661194), Stat3 (BD-Transduction Laboratories, S21320), Twist1/2 (Gene Tex, GTX127310), pY705-Stat3 (Cell Signaling Technology, #9131), Snail-1 (Cell Signaling Technology, LF062), CK5 (ABCAM, ab52635), pY418-Src (Invitrogen, 44660G), GAPDH (MAB374) and MMP9 (AB19016) (Millipore), PARP (Biomol, SA-249 clone C-2_10), β-actin (A5441), TAZ (HPA007415), and hydrocortisone were from Sigma-Aldrich, and CD44 (clone HP 2/9) was a gitf from Dr. F. Sanchez-Madrid (University Hospital La Princesa, UAM) [17 (link)], Matrigel^TM (Corning). Secondary horseradish peroxidase-conjugated antibodies, and B27 (Life Technologies). EGF, and bFGF (PeproTech EC Ltd). Fetal Calf Serum (FCS), Acrylamide/Bisacrylamide, SDS and ammonium persulfate (Bio-Rad Laboratories). ECL (GE Healthcare Biosciences). BCA protein assay (Thermo Scientific).

Mayoral-Varo V., Calcabrini A., Sánchez-Bailón M.P, & Martín-Pérez J. (2017). miR205 inhibits stem cell renewal in SUM159PT breast cancer cells. PLoS ONE, 12(11), e0188637.

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Western Blot Analysis of EMT Markers

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RIPA buffer with phosphatase and protease inhibitor was used to extract total protein in tissues or cells. Proteins were separated by SDS-PAGE and transferred onto the PVDF membrane. After blocking in 5% non-fat milk with PBST, primary antibodies against TEAD4 (GTX108750, GeneTex), E-cadherin (20,874-1-AP, Proteintech), N-cadherin (22,018-1-AP, Proteintech), Fibronectin (sc-8422, Santa Cruz), Twist1/2 (GTX127310, GeneTex) or GAPDH (sc-32,233, Santa Cruz) were used. The membranes were then incubated in the horseradish peroxidase (HRP)-conjugated secondary antibodies after washing in PBST three times. ECL substrate kit (Tanon Science & Technology, Shanghai, China) was used to visualize the immunoreactive bands on the blots.

Huang Z., Yan Y., Tang P., Cai J., Cao X., Wang Z., Zhang F, & Shen B. (2021). TEAD4 as a Prognostic Marker Promotes Cell Migration and Invasion of Urinary Bladder Cancer via EMT. OncoTargets and therapy, 14, 937-949.

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Evaluating Protein Expression and Modifications via Western Blotting

Cited 1 time

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Western blotting was performed as previously described(74 (link)). Nuclear and cytoplasmic fractions of cell lysates were isolated following manufacturer’s protocol (#78835, ThermoFisher). Protein concentrations were quantified using BCA assay (#23227, ThermoFisher). Around 20 μg of protein was loaded on 8% SDS-PAGE gels, transferred to PDVF membrane, blocked with 5% non-fat milk in 1xTBST, incubated with primary antibodies overnight at 4°C. Primary antibodies: DDR2 (1:1000, PA1879, Boster); phospho-DDR2 Y740 (1:1000, MAB25382, R&D systems); α-tubulin (1:1000, sc-32293, Santa Cruz); β-tubulin (1:1000, #2128, Cell Signaling); GAPDH (1:2000, sc-25778, Santa Cruz); Twist1/2 (1:1000, GTX127310, GeneTex); Snail (1:1000, #3895, Cell signaling; Lamin A/C (1:1000, #4777T, Cell signaling); TAZ (1:1000, 560235, BD biosciences); YAP (1:1000, sc376830, Santa Cruz); YAP (1:1000, #14074, Cell Signaling); E-cadherin (1:1000, 610404, BD biosciences). For immunoprecipitation, Phospho-(Ser/Thr) Phe Antibody (1:100, #9631, Cell Signaling).

Lin C.C., Yang W.H., Lin Y.T., Tang X., Chen P.H., Ding C.K., Qu D.C., Alvarez J.V, & Chi J.T. (2021). DDR2 upregulation confers ferroptosis susceptibility of recurrent breast tumors through the Hippo pathway. Oncogene, 40(11), 2018-2034.

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