Multicolor flow cytometric analysis was performed on isolated cells according to standard procedures using human mAbs that cross-react with PTM and RM. Cells were stained with Aqua Live/Dead amine dye (Invitrogen) to identify dead cells. Predetermined optimal concentrations were then used of the following antibodies: anti-CD3-Alexa700 (clone SP34-2, BDPharmigen), anti-CD8 PacBlue (clone RPA-T8, BDPharmigen), anti-CD4 PE-Cy5.5 (clone OKT4, eBioscience), anti-CD4 PE-Cy5.5 (clone L200, BDPharmigen), anti-Ki67 FITC (clone B26, BDPharmigen), anti-CCR5 PE (clone 3A9, BDPharmigen), anti-CD28 ECD (clone 28.2, Beckman Coulter), anti-CD95 PE-Cy5 (clone DX2, BDPharmigen), anti-CD14 APC (clone M5E2, BDPharmigen), All samples were permeabilized and fixed using CytoFix/Perm Kit (BDPharmigen) and intracellularly stained to detect Ki67. The acquired data were anlalyzed using FlowJo software (Treestar).
Anti cd28 ecd clone 28
Manufactured by Beckman Coulter
The Anti-CD28 ECD (clone 28.2) is a monoclonal antibody used for the detection and analysis of CD28 expression on the surface of cells. CD28 is a co-stimulatory receptor expressed on T cells that plays a crucial role in T cell activation and proliferation. This antibody is conjugated with Phycoerythrin-Texas Red-X (ECD), which allows for flow cytometric detection and quantification of CD28-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Live dead aqua amine dye, by Thermo Fisher Scientific (2 mentions)
Anti cd3 alexa700 clone sp34 2, by BD (2 mentions)
Anti cd8 pacblue clone rpa t8, by BD (2 mentions)
Anti cd4 pe cy5.5 clone okt4, by Thermo Fisher Scientific (2 mentions)
Anti cd4 pe cy5.5 clone l200, by BD (2 mentions)
Anti ki67 fitc clone b26, by BD (2 mentions)
Anti ccr5 pe clone 3a9, by BD (2 mentions)
Anti cd95 pe cy5, by BD (2 mentions)
Anti cd14 apc clone m5e2, by BD (2 mentions)
Cytofix perm kit, by BD (2 mentions)
2 protocols using anti cd28 ecd clone 28
1
Multicolor Flow Cytometry for T-Cell Phenotyping
2
Multicolor Flow Cytometry for T-Cell Phenotyping
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Multicolor flow cytometric analysis was performed on isolated cells according to standard procedures using human mAbs that cross-react with PTM and RM. Cells were stained with Aqua Live/Dead amine dye (Invitrogen) to identify dead cells. Predetermined optimal concentrations were then used of the following antibodies: anti-CD3-Alexa700 (clone SP34-2, BDPharmigen), anti-CD8 PacBlue (clone RPA-T8, BDPharmigen), anti-CD4 PE-Cy5.5 (clone OKT4, eBioscience), anti-CD4 PE-Cy5.5 (clone L200, BDPharmigen), anti-Ki67 FITC (clone B26, BDPharmigen), anti-CCR5 PE (clone 3A9, BDPharmigen), anti-CD28 ECD (clone 28.2, Beckman Coulter), anti-CD95 PE-Cy5 (clone DX2, BDPharmigen), anti-CD14 APC (clone M5E2, BDPharmigen), All samples were permeabilized and fixed using CytoFix/Perm Kit (BDPharmigen) and intracellularly stained to detect Ki67. The acquired data were anlalyzed using FlowJo software (Treestar).
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