Due to bulky shape of MIPs1 obtained using the first approach and an insufficient reaction yield of the second approach (MIPs2) an in situ MIPs polymerization, directly on the electrode surface was investigated. In situ polymerization was prepared with the same pre-polymerization mixture as used in the second approach, implementing two kinds of initiators, AIBN and Ciba® IRGACURE® 651 in order to choose the best method. Thermal polymerization (MIPs3) was initiated by addition of 2,2-azobis-2-isobutyronitrile (AIBN), and the reaction was carried out for 24 h under 60 °C. UV polymerization (MIPs4) was initiated by Ciba® IRGACURE® 651 (2,2-Dimethoxy-1,2-diphenylethan-1-one), and performed under UV lamps with UV wave length range between 300-400 nm, for 1 hour.
The morphologies of microspherical MIPs particles prepared by bulk, precipitation and in situ polymerization were compared using Scanning Electron Microscopy (SEM, FEI Company, Eindhoven
The Netherlands, (Fig. 1). For reference, these MIPs were compared with commercial MIPs towards amphetamine (Fig. 1d). The obtained functionalized electrodes were eventually tested using an electrochemical detection of N-FA performed using an automated flow injection system, developed by CapSenze AB (Lund, Sweden).
The morphologies of microspherical MIPs particles prepared by bulk, precipitation and in situ polymerization were compared using Scanning Electron Microscopy (SEM, FEI Company, Eindhoven
The Netherlands, (Fig. 1). For reference, these MIPs were compared with commercial MIPs towards amphetamine (Fig. 1d). The obtained functionalized electrodes were eventually tested using an electrochemical detection of N-FA performed using an automated flow injection system, developed by CapSenze AB (Lund, Sweden).