Phosphostop easypacks phosphotase inhibitors cocktail

The following reagents and antibodies were used for biological experiments: anisomycin (NeoCorp, Weilheim, Germany); annexin-V-FITC (BD Bioscience, San Jose, CA, USA); docetaxel (Pharmacy of the University Hospital Hamburg-Eppendorf, Hamburg, Germany); SP600125 (JNK1/2 inhibitor) and SB203580 (p38 inhibitor) (LC Laboratories, Woburn, MA, USA); PD98059 (MEK inhibitor) (Merck Chemicals GmbH, Darmstadt, Germany); PhosphoSTOP™ EASYpacks phosphotase inhibitors cocktail and cOmplete™ EASYpacks protease inhibitors cocktail (Roche, Mannheim, Germany); propidium iodide (PI), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma, Taufkirchen, Germany); RNase (Carl Roth, Karlsruhe, Germany); tariquidar (p-glycoprotein inhibitor) (MedChemExpress, Monmouth Junction, NJ, USA); primary and secondary antibodies used are listed in Table 2.

Docetaxel was purchased from Pharmacy of the University Hospital Hamburg-Eppendorf (Hamburg, Germany); PhosphoSTOP™ EASYpacks phosphotase inhibitors cocktail and cOmplete™ EASYpacks protease inhibitors cocktail were purchased from Roche (Mannheim, Germany); MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was purchased from Sigma (Taufkirchen, Germany); 3-Methyladenine was purchased from Enzo Life Sciences (Farmingdale, NY, USA); Tariquidar was purchased from MedChemExpress (Monmouth Junction, NJ, USA); Primary and secondary antibodies used for Western blotting are listed in Table 2.