Sodium chloride powder

All linear DNA templates were purchased from Integrated DNA Technologies (IA, USA). CircLigase II, CircLigase II buffer, Betaine (5M), MnCl₂, and nuclease-free water were purchased from Biosearch Technologies (Hoddesdon, UK). ATP (10 mM) and dNTPs (10 mM) were purchased from Thermo Scientific (MA, USA). Exonuclease I (10 U), Exonuclease III (100 U), NEB2 buffer (10×), BSA (20 mg/ml), and phi29 DNA polymerase (10 000 units/ml) were purchased from New England Biolabs (MA, USA). DTT (1M), SYBR Gold (10 000×), and ROX reference dye were purchased from Invitrogen (MA, USA). TE buffer (100×) and GelRed Nucleic Acid Stain (10 000× DMSO) were purchased from EMD Millipore (MA, USA). Nuclease-free water was purchased from Omega Bio-tek (GA, USA). Sodium chloride powder and Tween-20 were purchased from Sigma Aldrich (USA).

Gelatin from bovine skin (type B) and alginic acid sodium salt, dimethyl sulfoxide (DMSO), calcium chloride, and sodium chloride powder were purchased from Sigma-Aldrich (Canada). calcium chloride was dissolved in Millipore water at a final concentration of 4%, filtered with 0.2 u filters and stored in a sterile condition for further use. For cell culture studies, MCF7 and MDA-MB-231 cell lines were purchased from ATCC (Cedarlane, Canada). DMEM (Dulbecco’s Modified Eagle’s Medium, without sodium pyruvate, with 4.5 g/l glucose, with L-glutamine), PBS (phosphate buffer saline) 1×, Penicillin-streptomycin, Trypsin/EDTA solution at 0.25% (w./v.) and FBS (fetal bovine serum) were purchased from Wisent Bioproducts (Canada). (3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H-tetrazolium bromide) MTT powder, Triton X-100, BSA (bovine serum albumin), paraformaldehyde, a Live/Dead Cell Viability assay kit (CBA415) and Hoechst 33342 Nuclei Dye, Propidium Iodide (PI) and Calcine were bought from Sigma-Aldrich (Canada). For bioprinting, the mixing syringe (5ml) with the mixing ratio of 4:1, and compatible mixer tips were purchased from Sulzer Inc. (Switzerland). Empty cartridges and nozzles purchased from Cellink (Sweden)