Anti phospho fak tyr397

Anti-SCGN antibody was from AbFrontier. Anti-FAK, anti-paxillin, anti-phospho-paxillin (Tyr¹¹⁸), anti-ERK1/2, anti-phospho-ERK1/2 (Thr²⁰²/Tyr²⁰⁴), anti-Akt and anti-phospho-Akt (Ser⁴⁷³) antibodies were from Cell Signaling Technology. Anti-α-tubulin antibody, anti-β-actin antibody and normal rabbit IgG were from Santa Cruz Biotechnology. Anti-phospho-FAK (Tyr³⁹⁷) and anti-SCGN antibodies used in immunoprecipitation were from Abcam. anti-paxillin antibody used in confocal microscopy was from Millipore Corporation. Anti-E-cadherin (epithelial cadherin) and anti-N-cadherin (neural cadherin) antibodies were from BD Biosciences. Horseradish peroxidase-conjugated goat anti-mouse IgG and goat anti-rabbit IgG were from Bio-Rad Laboratories. Rhodamine–phalloidin, Alexa Fluor® 488- or Alexa Fluor® 568-conjugated goat anti-rabbit IgG and Alexa Fluor® 488-conjugated goat anti-mouse IgG were from Invitrogen. Latrunculin B was from Calbiochem. Cytochalasin D, ionomycin and DMSO from Sigma–Aldrich. Penicillin G, streptomycin, FBS and trypsin were from Gibco Life Technologies. DMEM (Dulbecco's modified Eagle's medium) and 45% D-glucose were from WelGENE. SMARTpool siRNA and DharmaFECT1 transfection reagent were from Dharmacon. Insulin ELISA kit was from ALPCO. BCA protein assay was from Thermo Scientific. Protein G–Sepharose beads and silver staining kit were from GE Healthcare.