Pme 1

The following antibodies were used at the indicated dilutions: PME‐1, Santa Cruz Biotechnology (Dallas, TX, USA) sc‐20086 (H‐226), Western blotting 1 : 1000; PME‐1, Santa Cruz Biotechnology sc‐25278 (B‐12), Immunohistochemistry 1 : 1000, Immunofluorescence 1 : 100; cleaved PARP‐1, Abcam (Cambridge, UK) ab32064 [E51], Western blotting 1 : 1000; GAPDH, HyTest 5G4‐6C5, Western blotting 1 : 5000; c‐MYC, Abcam ab32072 [Y69], Western blotting 1 : 1000; p‐Myc S62, Abcam ab78318, Western blotting 1 : 1000; AKT1/2/3, Cell Signaling Technology #9272, Western blotting 1 : 2000; p‐AKT S473, Cell Signaling Technology #4060, Western blotting 1 : 1000; Lamin‐A/C, Santa Cruz Biotechnology sc‐7292 (636), Immunofluorescence 1 : 250; Lamin‐A/C, Santa Cruz Biotechnology sc‐6215 (N‐18), Western blotting 1 : 1000; p‐Lamin‐A/C S392, Abcam ab58528, Western blotting 1 : 5000; EEA1, Santa Cruz Biotechnology sc‐137130 [G4], Immunofluorescence 1 : 100; p‐FAK Y397, Cell Signaling Technology #8556, Immunofluorescence 1 : 100; Histone H3K9me3, Cell Signaling Technology #13969 [D4W1U], Immunofluorescence 1 : 500; Histone H3K27me3, Cell Signaling Technology #9733 [C36B11], Immunofluorescence 1 : 500; PPP2R2A, Cell Signaling Technology #5689, Western blotting 1 : 1000.

Phorbol 12-myristate 13-acetate (PMA, P8139, purity > 99%), taurine powder (T0625, purity > 99%), ABL127 (SML0294, purity > 98%), Lipopolysaccharides (LPS, L2630), Methionine-free medium (RPMI-1640 medium, R7513), and methionine powder (L-Methionine, M9625, purity > 98%) were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). Cell culture medium (RPMI-1640 medium, 3180022) was purchased from Gibco-Life Technologies (Grand Island, USA). FastStart Universal SYBR Green Master Mix (ROX) (4913914001) was obtained from Roche (Basel, Switzerland); Hexokinase (HK) Assay Kit (BC0680), pyruvate kinase (PK) Assay Kit (BC0545), Lactate dehydrogenase (LDH) Assay Kit (BC0685), Pyruvic acid dehydrogenase (PDH) Assay Kit (BC0385) and Neutral Red solution, 0.33% (G1310) were purchased from Solarbio (Shanghai, China); ATP assay kit (S0026), Mito-Tracker Green (C1048), Mitochondrial membrane potential assay kit with JC-1 (C2006), Lyso-Tracker Red (C1046) and α-Tubulin were obtained from Beyotime (Jiangsu, China). Beclin-1, Cryopyrin (NLRP3), PINK1, Parkin, demethylated PP2Ac, methylated PP2Ac, LCMT-1, PME-1, total PP2Ac antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-LC3B antibodies were purchased from Abcam Co. (Cambridge, UK). SQSTM1 (p62) and Bafilomycin A1 (BAF) (54645) were purchased from Cell Signaling Technology Co. (Danvers, MA).

Cell lysates were prepared and separated by SDS-PAGE as previously described^{25 (link)}. Proteins were transferred onto nitrocellulose membranes (Bio-Rad). Membranes were blocked with 5% milk in TBS-T, followed by primary antibody incubation overnight at 4 °C. Primary antibodies: PME-1 (Santa Cruz, sc-20086, 1:1000), phospho Akt S473 (Cell Signaling, 9271, 1:1000), phospho PDHE1α S300 (Millipore, ABS194, 1:1000), β-actin (Sigma-Aldrich, A1978, 1:10,000) and GAPDH (HyTest, 5G4cc, 1:10,000). Secondary antibodies were purchased from LI-COR Biotechnology or Dako (Agilent Technologies). Membranes were scanned using an Odyssey Imager (LI-COR Biotechnology) or HRP antibodies were detected using an ECL-based Curix 60 film processor (Agfa).