COS7 cells were transfected with either pDEST53-PKCδ or pDEST53-PKCδ-IVMEF, and after 48 h cells were exposed to vehicle, 50 nM rapamycin, or 250 U/ml IFN-β for the indicated times before incubation with a PE-AnnexinV and 7-amino-actinomycin D (7-AAD) Apoptosis Detection Kit I (BD Biosciences) as per manufacturer’s instructions. Flow cytometry was performed using BD Fortessa X-20 (BD Biosciences) with FACSDiva Software (BD Biosciences). Subsequent analysis was performed using FlowJo Software Version 10.2 (Tree Star).
Pe annexinv and 7 amino actinomycin d 7 aad apoptosis detection kit 1
Manufactured by BD
The PE-Annexin V and 7-amino-actinomycin D (7-AAD) Apoptosis Detection Kit is a laboratory tool used to identify and quantify apoptotic cells. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and 7-AAD, a DNA-binding dye, to differentiate between viable, early apoptotic, and late apoptotic/necrotic cells.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using pe annexinv and 7 amino actinomycin d 7 aad apoptosis detection kit 1
1
Apoptosis Assay in COS7 Cells
2
Multiparametric Viability Assay for Viral Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LIVE/DEAD Fixable Violet Dead Cell staining was implemented to determine cell viability following viral infection (Molecular Probes). Necrosis and apoptosis levels in vitro and in vivo were assessed using the PE-AnnexinV and 7-amino-actinomycin D (7-AAD) Apoptosis Detection Kit I (BD Biosciences) according to the manufacturer’s instructions and analyzed by flow cytometry. Lactate dehydrogenase (LDH) release in culture supernatants of IAV-infected BMD-Mφ was quantified using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega), as per the manufacturer’s recommendations.
3
Quantifying Apoptosis in Alveolar Macrophages
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Necrosis and apoptosis levels of BAL AMs were assessed using a PE-AnnexinV and 7-amino-actinomycin D (7-AAD) Apoptosis Detection Kit I (BD Biosciences) according to the manufacturer’s instructions and analysed by flow cytometry.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!