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Image studio software

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Image Studio software is a data analysis tool for digital imaging and quantification of gel and blot data. It provides a user-friendly interface for processing and analyzing images captured with Bio-Rad imaging systems.

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Lab products found in correlation

Image lab software, by Bio-Rad (2 mentions) Prism 6, by GraphPad (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Irdye 800cw goat anti rabbit igg, by LI COR (1 mentions) Irdye 680rd goat anti mouse igg, by LI COR (1 mentions) Ripa buffer, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Odyssey blocking buffer tbs, by LI COR (1 mentions) Mini protean tetra system, by Bio-Rad (1 mentions) Dc detergent compatible protein assay, by Bio-Rad (1 mentions) Licor image studio lite, by Bio-Rad (1 mentions)

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Image Studio (3 citations)

4 protocols using image studio software

1

Imaging and Analysis of Apoptosis

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Data collection for immunofluorescent images was performed using Columbus image analysis program (PerkinElmer) as described in (15 ) or Fiji image analysis software (43 (link)). Caspase-3/7 analysis and data collection was performed using Essen BioScience IncuCyte Zoom kinetic cell imager and the related software. Densitometry of immunoblots was analyzed using LI-COR Image Studio software and BioRad Image Lab software. Where appropriate, data were analyzed using one-tailed unpaired Student t test with Welch’s correction using the GraphPad Prism 6 software and are expressed as a mean ± SEM of at least three independent experiments.
Bhattarai K., Richard T., Fatica T., Frangione B., Willmore W.G, & Holcik M. (2022). AMPK-related protein kinase ARK5 regulates subcellular localization of RNA-binding protein hnRNP A1 during hypertonic stress. The Journal of Biological Chemistry, 298(9), 102364.
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2

Automated Microscopy-based Cell Death Analysis

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YOYO-1 and caspase 3/7 data collection and analysis were performed using the Essen IncuCyte Zoom S3 kinetic cell imager and the IncuCyte software v 2020B. Densitometry of immunoblots and RNA gels was analyzed using LI-COR® Image Studio software and BioRad® Image Lab software v 6.1.0 build 7. Where appropriate, data were analyzed using a one-tailed unpaired Student t-test using the GraphPad Prism software v 7.01 and are expressed as a mean ± standard deviation of at least three independent experiments.
Fatica T., Naas T., Liwak U., Slaa H., Souaid M., Frangione B., Kattini R., Gaudreau-Lapierre A., Trinkle-Mulcahy L., Chakraborty P, & Holcik M. (2023). TRNT-1 Deficiency Is Associated with Loss of tRNA Integrity and Imbalance of Distinct Proteins. Genes, 14(5), 1043.
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3

Western Blot Analysis of Protein Expression

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Cells were detached with trypsin-EDTA (0.05%) (Gibco), washed with PBS (Gibco), and lysed in RIPA buffer (Thermo Fisher) supplemented with protease inhibitor cocktail and phosphatase inhibitor cocktail tablets (Roche). Cleared supernatants were subjected to protein quantification by DC (detergent compatible) protein assay (Bio-Rad). Western blot was performed using the Bio-Rad Mini-PROTEAN Tetra System. Proteins were resolved by SDS-PAGE (4–15%), transferred to 0.45 µM nitrocellulose membranes, and blocked in 1:1 TBS Odyssey Blocking Buffer (LI-COR) and 1X TBST for 1 hour at room temperature. Membranes were incubated rotating overnight at 4°C with primary antibody. See Supplementary Materials for list of antibodies and dilutions. Membranes were then washed 4 times for 5 minutes each in TBST and probed with secondary antibody IRDye 680RD Goat anti-Mouse IgG 1:15,000, IRDye 800CW Goat anti-Rabbit IgG 1:10,000 (LI-COR), or donkey anti-Rabbit IgG peroxidase-linked secondary (Cytiva) 1:10,000. Quantification was performed using LI-COR Image Studio software or Bio-rad Image Lab Software.
Jiménez J.A., Apfelbaum A., Hawkins A.G., Svoboda L.K., Kumar A., Ocadiz Ruiz R., Garcia A., Haarer E., Nwosu Z.C., Bradin J., Purohit T., Chen D., Cierpicki T., Grembecka J., Lyssiotis C.A, & Lawlor E.R. (2021). EWS-FLI1 and Menin Converge to Regulate ATF4 Activity in Ewing sarcoma. Molecular cancer research : MCR, 19(7), 1182-1195.
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4

Western Blot Data Quantification Protocol

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Western blot data was quantified using the Li-Cor image studio lite or Bio-Rad Image Studio software. Samples were normalized to a loading control (VCP, synaptophysin, or GAPDH) before being normalized to the average of all WT (Cre-negative tamoxifen-treated) samples within the same gel. Note that loading controls used for subcellular fractionation (synaptophysin and GAPDH) are present (though not enriched) in all fractions analyzed.82 (link),83 (link)
Mendoza M.L., Quigley L.D., Dunham T, & Volk L.J. (2022). KIBRA regulates activity-induced AMPA receptor expression and synaptic plasticity in an age-dependent manner. iScience, 25(12), 105623.
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