Bruker AV-300 NMR spectrometer was applied for characterizing 1H NMR spectra. Gel permeation chromatography (GPC) measurements were conducted on a Waters GPC system. The eluant was DMF (containing 0.01 M LiBr) with a flow rate of 1.0 ml/min and monodisperse polystyrene as standard samples. The amount of PTX of drug release experiment and biodistribution experiment were measured by high performance liquid chromatography (HPLC) which was conducted via a PerkinElmer Flexar system. Dynamic light scattering (DLS) which was performed on Malvern Zetasizer instrument (Nano-ZS90) was used to characterize the self-assemble and the sizes of NPs. A JEOL JEM-1011 transmission electron microscopy (TEM, Tokyo, Japan) was used to obtain the morphology images of the self-assemble NPs. Optical microscope (Nikon Eclipse Ti, Optical Apparatus Co., Ardmore, PA, USA) was used to observe histological alterations. The immunofluorescence slides were imaged on a Carl Zeiss LSM 700 confocal laser scanning microscope (CLSM).
Jem 1011 transmission electron microscopy
Manufactured by JEOL
Sourced in Japan
The JEM-1011 is a transmission electron microscope manufactured by JEOL. It is designed to provide high-resolution imaging of samples at the nanoscale level. The JEM-1011 utilizes an electron beam to illuminate and interact with a thin specimen, allowing users to observe the detailed structure and composition of materials.
Automatically generated - may contain errors
Lab products found in correlation
Av 300 nmr spectrometer, by Bruker (1 mentions)
Nano zs90, by Malvern Panalytical (1 mentions)
Flexar system, by PerkinElmer (1 mentions)
Confocal laser scanning microscope lsm700, by Zeiss (1 mentions)
Gpc system, by Waters Corporation (1 mentions)
Optima 5300 dv icp oes, by PerkinElmer (1 mentions)
Sigma 300 sem, by Zeiss (1 mentions)
2600 uv visible spectrophotometer, by Shimadzu (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Flow cytometry, by Beckman Coulter (1 mentions)
4 protocols using jem 1011 transmission electron microscopy
1
Comprehensive Characterization of Self-Assembled Nanoparticles
2
Comprehensive Characterization of nZVI and E. Coli
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A laboratory Shanghai Leici pHS-3C pH meter (Shanghai, China) was used to monitor pH value. The morphological analysis of nZVI, biochar, and BC-nZVI samples were performed by a JEOL JEM-1011 transmission electron microscopy (TEM) (Tokyo, Japan), a FEI Quanta 600 scanning electron microscopy (SEM) (Eindhoven, the Netherlands), and a Carl Zeiss SIGMA 300 SEM (Oberkochen, Germany), respectively. The morphological analysis of E. Coli cells were performed by a JEOL JEM-1011 transmission electron microscopy (TEM) (Tokyo, Japan). The crystal morphology of nZVI samples were analyzed by Rigaku DXR-8000 X-ray diffraction (Tokyo, Japan). Concentrations of Fe(ii ) were measured at 510 nm by the Shimadzu UV-visible 2600 spectrophotometer (Kyoto, Japan). Standards containing known concentrations of ferrous ion were used as reference for each set of tests. Concentrations of Se(vi ) in solution were measured by the PerkinElmer Optima 5300 DV ICP-OES (Waltham, United States). Contents of protein and SOD in cells by Coomassie brilliant blue G250 and SOD kit.
3
Apoptosis Detection by DUTP-FITC/PI Staining
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DUTP-FITC/propidium iodide (PI) staining was performed for the detection of apoptotic cells. 48 h after transfection, 1×106 cells were collected and washed twice with ice-cold PBS (pH = 7.2, GIBCO, Grand Island, NY, USA). The cells were stained using the 0.002% Triton X-100, 100 U/ml RNase and 50 µg/ml PI for 30 min at 37°C, and then were detected by flow cytometry according to the guidelines. The non-transfected cells served as a negative control. The analysis of cell cycles and cell apoptosis was measured by flow cytometry (Beckman-Coulter, CA, USA), and DNA content was analyzed by COULT WINCYCLE software. The cell cycle distribution and cell percentage at G0/G1, G2/M and S phase were determined by Wincycle DNA software. More than 1×106 cells were fixed with 3.5% glutaraldehyde following with 1% osmic acid. Then cells were dehydrated by increasing alcohol and acetone concentration gradually and embedded with embedding medium. The semithin section of cell samples was measured using ultra-microtome and staining with the citrate-uranium acetate. Finally, images were collected under the JEM-1011 transmission electron microscopy (JEOL, Tokyo, Japan).
4
Characterization of VO2 Nanoparticles
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The morphology and size of the VO2 nanoparticles were acquired using a transmission electron microscopy (TEM) by JEM-1011 transmission electron microscopy (JEOL, Tokyo, Japan) with a working voltage at 100 kV. The X-ray powder diffraction method was carried out in a D/max-rα power diffractometer (Rigaku, Tokyo, Japan) using Cu-Kα monochromatic radiation (λ = 1.5418 Å).
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