Big dye terminator abi prism kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Big Dye Terminator ABI Prism Kit is a DNA sequencing reagent kit produced by Thermo Fisher Scientific. The kit contains the necessary components for performing DNA sequencing using the Sanger sequencing method.

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Lab products found in correlation

Sequencer package, by Gene Codes (2 mentions) 3730 genetic analyser, by Thermo Fisher Scientific (1 mentions) Autopure ls, by Qiagen (1 mentions) Abi prism 3700 dna analyser, by Thermo Fisher Scientific (1 mentions) Autopure ls system, by Qiagen (1 mentions) 3730 genetic analyzer, by Thermo Fisher Scientific (1 mentions)

3 protocols using big dye terminator abi prism kit

Genetic Mutation Identification in Dyslipidemia

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Genomic DNA was prepared from 5 mL of whole blood on an AutopureLS apparatus according to a protocol provided by the manufacturer (Gentra Systems, Minneapolis, MN). Mutation identification in the LDLR, APOB, and PCSK9 genes was performed by direct Sanger sequencing; identification of large rearrangements in the LDLR gene was done by multiplex ligation-dependent probe technique as described previously in more detail.^{25 (link)}. Sequence analysis was performed by direct sequencing with the Big Dye Terminator ABI Prism Kit, version 1.1 (Applied Biosystems, Foster City, CA). Products of sequence reactions were run on a genetic analyser 3730 (Applied Biosystems), and sequence data were analysed using the sequencer package (GeneCodes Co, Ann Arbor, MI). Mutations were described according to the nomenclature proposed by den Dunnen and Antonarakis.^{26 (link)}

de Boer L.M., Hutten B.A., Zwinderman A.H, & Wiegman A. (2022). Lipoprotein(a) levels in children with suspected familial hypercholesterolaemia: a cross-sectional study. European Heart Journal, 44(16), 1421-1428.

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Genetic Profiling of PAH Patients

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Genomic DNA was isolated from peripheral blood leukocytes of PAH patients. Polymerase chain reaction (PCR) was used to amplify the exons and flanking intronic bases of the five genes, including BMPR2 (13 exons), ALK-1 (10 exons), CAV1 (3 exons), ENG (14 exons), and KCNK3 (2 exons). The primers used for PCR were designed using reference sequences deposited in the GenBank database. Standard DNA sequencing reactions were performed using the fluorescence-labelled dideoxy chain termination method with the BigDye Terminator ABI Prism Kit and the ABI PRISM™ 3700 DNA Analyser (Applied Biosystems, Foster City, CA, USA), according to the manufacturer’s instructions.

Wang M.T., Charng M.J., Chi P.L., Cheng C.C., Hung C.C, & Huang W.C. (2021). Gene Mutation Annotation and Pedigree for Pulmonary Arterial Hypertension Patients in Han Chinese Patients. Global Heart, 16(1), 70.

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Genetic Screening for LDLR and APOB

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Genomic DNA was isolated from 5 mL whole blood on an AutopureLS system according to a protocol provided by the manufacturer (Gentra Systems, Minneapolis, MN). Mutation identification in the LDLR and APOB genes was performed by direct Sanger sequencing; identification of large rearrangements in the LDLR gene was done by multiplex ligation-dependent probe technique. Sequence analysis was performed by direct sequencing with the Big Dye Terminator ABI Prism Kit, version 1.1 (Applied Biosystems, Foster City, CA). Products of sequence reactions were run on a Genetic Analyzer 3730 (Applied Biosystems), and sequence data were analyzed by the use of the Sequencer package (GeneCodes Co, Ann Arbor, MI). We did not perform LPA gene analysis in the current study.

de Boer L.M., Hof M.H., Wiegman A., Stroobants A.K., Kastelein J.J.P, & Hutten B.A. (2022). Lipoprotein(a) levels from childhood to adulthood: Data in nearly 3,000 children who visited a pediatric lipid clinic. Atherosclerosis, 349.

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