Diethylnitrosamine (DEN) and aspirin were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Antibodies against IRS1, phospho- IRS-1(Ser1101), NF-kappaB, phospho-NF-kappaB, AKT, phospho-AKT(Ser473), phosphor-AKT (Thr308), JNK, phospho-SAPK/JNK (Thr183/Tyr185), phospho-p38MARK (Thr180/Tyr182), phospho-p44/42 MARK (Erk1/2) (Thr202/Tyr204), AMPK-α, phospho-AMPK-α(Thr172),ACC, phospho-ACC(Ser79),β-Catenin, phospho-β-Catenin (Ser33/37/Thr41) and GAPDH were purchased from Cell Signaling Technology (USA). Antibodies against Srebp1c, Glut4, Glut2 were from Santa Cruz Biotechnology Inc. (USA). The antibody against PPAR-α was acquired from Millipore Sigma (USA). The antibody against PPAR-γ was obtained from Abcam Co. (USA). The bicinchonic acid (BCA) protein assay kit and the phosphatase inhibitor tablets were purchased from Thermo Fisher Scientific (USA). Concentrations of the primary and secondary antibodies are described in the supplemental table .
Phospho nf kappab
Manufactured by Cell Signaling Technology
Sourced in United States
Phospho-NF-kappaB is a laboratory product used to detect and quantify the phosphorylated form of the NF-kappaB protein. NF-kappaB is a transcription factor that plays a key role in regulating the immune response and inflammation. The phospho-NF-kappaB product can be used in various research applications to study the activation and signaling of this important cellular pathway.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh, by Cell Signaling Technology (3 mentions)
Phosphatase inhibitor tablet, by Thermo Fisher Scientific (2 mentions)
Antibody against ppar α, by Merck Group (2 mentions)
Aspirin, by Merck Group (2 mentions)
Phospho p44 42 mark erk1 2 thr202 tyr204, by Cell Signaling Technology (2 mentions)
Antibody against ppar γ, by Abcam (2 mentions)
Phospho β catenin ser33 37 thr41, by Cell Signaling Technology (2 mentions)
Bicinchonic acid bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Diethylnitrosamine den, by Merck Group (2 mentions)
Ampkα, by Cell Signaling Technology (2 mentions)
F4 80, by Cell Signaling Technology (1 mentions)
Phospho sapk jnk thr183 tyr185, by Cell Signaling Technology (1 mentions)
Phospho akt 308, by Cell Signaling Technology (1 mentions)
Nf kappab, by Cell Signaling Technology (1 mentions)
Pparg, by Cell Signaling Technology (1 mentions)
Phospho mtor, by Cell Signaling Technology (1 mentions)
3 protocols using phospho nf kappab
1
Molecular Mechanisms in Liver Disease
2
Antibody-based Signaling Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies against mTOR, phospho-mTOR, IRS2, AKT, phospho-AKT-308, NF-kappaB, phospho-NF-kappaB, JNK, phospho-SAPK/JNK (Thr183/Tyr185), PPAR-g, F4/80, SREBP, and GAPDH were purchased from Cell Signaling Technology (USA).
3
Molecular Mechanisms in Liver Disease
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