Polyinosinic–polycytidylic acid sodium salt (Poly I:C) was purchased from Sigma-Aldrich, and recombinant human interleukin (IL)-1α and recombinant human IL-4 were purchased from PeproTech (Rocky Hill, NJ, USA). For western blot analysis, IL-4, IL-1β, IL-6, filaggrin (FLG), and loricrin (LOR) were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Human IL-4, IL-13, and enzyme-linked immunosorbent assay (ELISA) kits were purchased from R&D Systems (Minneapolis, MN, USA).
Recombinant human interleukin il 1α
Manufactured by Thermo Fisher Scientific
Sourced in United States
Recombinant human interleukin (IL)-1α is a protein that plays a key role in the immune and inflammatory response. It functions as a pro-inflammatory cytokine, promoting the activation and differentiation of various immune cells.
Automatically generated - may contain errors
Lab products found in correlation
Anti glyceraldehyde 3 phosphate dehydrogenase gapdh, by Santa Cruz Biotechnology (1 mentions)
Enzyme linked immunosorbent assay elisa kit, by R&D Systems (1 mentions)
Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions)
Recombinant human il 4, by Thermo Fisher Scientific (1 mentions)
Human il 4, by R&D Systems (1 mentions)
Il 1β, by Thermo Fisher Scientific (1 mentions)
Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions)
Polyinosinic polycytidylic acid sodium salt poly 1 c, by Merck Group (1 mentions)
Il 13, by R&D Systems (1 mentions)
Ficoll hypaque density gradient, by Cytiva (1 mentions)
Recombinant human il 2, by Thermo Fisher Scientific (1 mentions)
Ficoll paque plus, by Cytiva (1 mentions)
Anti cd3 antibody, by BioLegend (1 mentions)
2 protocols using recombinant human interleukin il 1α
1
Immune Modulation with Poly I:C and Cytokines
2
Autologous CIK Cell Preparation Protocol
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Autologous CIK cells were prepared as previously described [14 (link)]. Briefly, peripheral blood mononuclear cells (PBMCs) from participants were obtained from whole blood by centrifugation over Ficoll-Hypaque density gradient (Ficoll-Paque Plus; Amersham Biosciences). The PBMCs (2 × 106 cells/mL) were incubated in fresh RMPI-1640 medium (HyClone, USA) containing 10% serum and 1000 U/mL recombinant human gamma-interferon (IFN-γ, PeproTech, USA). After incubation for 24 h, anti-CD3 antibody (100 ng/mL, BioLegend, USA), recombinant human interleukin- (IL-) 1α (100 U/mL, PeproTech, USA), and recombinant human IL-2 (1000 U/mL, PeproTech, USA) were added to the medium. Cells were incubated and fed every 2 days in fresh complete medium supplemented with rhIL-2 (1000 U/mL) and maintained at a density of 2 × 106 cells/mL.
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