SDS-PAGE and Western blotting were carried out as described (18 ), except that conventional 12% Laemmli gels were used instead of Bis-Tris gel buffer. After electrophoresis was performed, the gels were blotted to nitrocellulose membranes using a Transblot Turbo apparatus (Bio-Rad) and developed using rabbit antisera raised against the ternary Ric complex, commercial anti-FLAG M2 monoclonal antiserum (Sigma) or a rabbit antiserum raised against Rny, a gift from Jörg Stulke. The signals were visualized using ECL Prime Western blotting detection reagent (GE Healthcare).
Anti flag m2 monoclonal antiserum
Manufactured by Merck Group
The Anti-FLAG M2 monoclonal antiserum is a laboratory reagent used for the detection and purification of proteins tagged with the FLAG epitope. It is a highly specific antibody that binds to the FLAG tag, which is a short peptide sequence commonly used to facilitate the identification and isolation of recombinant proteins.
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2 protocols using anti flag m2 monoclonal antiserum
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SDS-PAGE and Western Blot Protocol
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SDS-PAGE and Western Blotting Protocol
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SDS-PAGE and Western blotting were carried out as described (18 (link)), except that conventional 12% Laemmli gels were used instead of Bis-Tris gel buffer. After electrophoresis was performed, the gels were blotted to nitrocellulose membranes using a Transblot Turbo apparatus (Bio-Rad) and developed using rabbit antisera raised against the ternary Ric complex, commercial anti-FLAG M2 monoclonal antiserum (Sigma) or a rabbit antiserum raised against Rny, a gift from Jörg Stulke. The signals were visualized using ECL Prime Western blotting detection reagent (GE Healthcare).
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