C18 reverse phase lc hypersil gold column

Samples were analysed using a C18 Reverse-Phase LC Hypersil Gold Column, Thermofisher Scientific, on an Agilent 1100 series HPLC or Dionex UltiMate 3000 at 23 °C. 100 µl of organic solvent-quenched reaction supernatant was injected onto the column with isocratic mobile phases of 80% acetonitrile and 20% water with 0.1% phosphoric acid for fenpyroximate, pyridaben, tolfenpyrad, fenazaquin and deltamethrin, and 85% methanol and 15% water for DDT. Flow rates were 1 ml/min and monitoring absorbance wavelength was 226 nm apart from DDT, which was 232 nm. Elution times for the insecticides were as follows: fenpyroximate, 7.7 min; tolfenpyrad, 6.0 min; fenazaquin, 7.9 min; pyridaben, 9.3 min; deltamethrin, 9.8 min and DDT, 10.8 min. The insecticide was quantified by peak integration (OpenLAB Chromatography Data System). Examples of chromatograms used for peak quantification and quantitative assessment of P450 metabolism are illustrated in Supplementary Fig. 2.

Samples were analysed using a C18 Reverse-Phase LC Hypersil Gold Column, Thermofisher Scientific, on an Agilent 1100 series HPLC or Dionex UltiMate 3000 at 23 °C. 100 µl of organic solvent-quenched reaction supernatant was injected onto the column with isocratic mobile phases of 80% acetonitrile and 20% water with 0.1% phosphoric acid for fenpyroximate, pyridaben, tolfenpyrad, fenazaquin and deltamethrin, and 85% methanol and 15% water for DDT. Flow rates were 1 ml/min and monitoring absorbance wavelength was 226 nm apart from DDT, which was 232 nm. Elution times for the insecticides were as follows: fenpyroximate, 7.7 min; tolfenpyrad, 6.0 min; fenazaquin, 7.9 min; pyridaben, 9.3 min; deltamethrin, 9.8 min and DDT, 10.8 min. The insecticide was quantified by peak integration (OpenLAB Chromatography Data System). Examples of chromatograms used for peak quantification and quantitative assessment of P450 metabolism are illustrated in Supplementary Fig. 2.