The cultured cells in 500 mL of medium were collected by centrifugation (8000 g, 10 min) and washed twice using deionized water. The cells were lyophilized (laboratory-scale) or heated at 100 °C (pilot scale) to measure their dry mass. The dried cells (approximately 50 mg) were disrupted using mortar and pestle. Disrupted cells were suspended in 8 mL n-hexane for oil extraction. The hexane fraction was recovered using centrifugation (1000 g, 10 min) and evaporated using an argon gas spray. Finally, the hexane extract material was determined by weighing. PCA was performed with statistical computing software, namely, R (ver3.2.5) (developed by the R Development Core Team, The R Foundation, Auckland, New Zealand).
R ver3.2.5
Manufactured by R Project for Statistical Computing
Sourced in New Zealand, United States
R is a free software environment for statistical computing and graphics. It provides a wide variety of statistical and graphical techniques, and is highly extensible. R is a language and environment for statistical computing and graphics.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using r ver3.2.5
1
Lipid Extraction and Dry Mass Analysis
2
Statistical Analysis of Survival Outcomes
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Factors in the older and control groups were compared using Student t-tests for continuous variables and χ2 tests for categorical variables. The Kaplan-Meier method was used to estimate OS and CR rates. Variables for which P < 0.05 in univariate analysis were entered into a multivariate Cox-proportional hazards regression model to determine risk factors independently associated with adverse outcomes. Odds ratios and 95% confidence intervals (CIs) were calculated. All statistical analyses used IBM SPSS Statistics ver. 22.0 (IBM Co., Armonk, NY, USA) and R ver. 3.2.5 (R Foundation, Vienna, Austria).
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