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2 protocols using anti phosphor ampk

1

Molecular Mechanisms of Stem Cell Regulation

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Hepatocyte growth factor (HGF) and transforming growth factor β 1 (TGF-β-1) were purchased from Pepro Tech (Rocky Hill, USA). Rapamycin and AICAR were purchased from Calbiochem (San Diego, CA, USA). The anti-CD90 conjugated PE antibody was purchased from eBioscience (San Diego, CA, USA). The anti-EpCAM antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-mTOR and anti-CD90 antibodies were purchased from Epitomics (Burlingame, CA, USA). The anti-CD133 and anti-CD44 antibodies were purchased from Abcam (Cambridge, UK). The anti-phospho-mTOR, anti-phosphor-AMPK, anti-AMPK, anti-mTOR, secondary horse anti-mouse horseradish peroxidase-conjugated and anti-rabbit horseradish peroxidase-conjugated antibodies were purchased from Cell Signaling (Beverly, MA, USA). The anti-β-actin antibody was purchased from Chemicon (Pittsburgh, PA, USA). The AC133 antibody was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).
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2

Western Blot Analysis of Cellular Proteins

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Western blot analysis was performed as previously defined [24 (link)]. The primary antibodies anti-phosphor-ATM, anti-Bcl2, and anti-phosphor-AMPK antibodies (Cell Signaling Technology, Danvers, MA, USA) were used; anti-γH2AX, anti-mHtt, anti-V5, and anti-actin antibodies (Millipore, Billerica, MA, USA) were used. Immunoreactive signals on the blots were identified using an enhanced chemiluminescence (ECL) detection system (PerkinElmer Life and Analytical Sciences, Boston, MA, USA). The protein band quantifications were analyzed using the ImageJ Gel Analysis program.
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