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Amlexanox

Manufactured by Selleck Chemicals
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Sourced in United States

Amlexanox is a chemical compound used in laboratory research. It functions as an anti-inflammatory and immune modulator agent. No further details on intended use are provided.

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Lab products found in correlation

Wortmannin, by Selleck Chemicals (1 mentions) Anti syk, by Cell Signaling Technology (1 mentions) Anti traf6, by Cell Signaling Technology (1 mentions) Jnk inhibitor sp600125, by Selleck Chemicals (1 mentions) Anti myd88, by Cell Signaling Technology (1 mentions) Anti p jnk thr183 tyr185, by Cell Signaling Technology (1 mentions) Cell lysis buffer, by Cell Signaling Technology (1 mentions) Chip grade protein g magnetic beads, by Cell Signaling Technology (1 mentions) Anti flag, by Proteintech (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti p65, by Cell Signaling Technology (1 mentions) Anti p p65 ser536, by Cell Signaling Technology (1 mentions) Anti lamin a c, by Proteintech (1 mentions) Anti myc, by Proteintech (1 mentions) Bay11 7082, by R&D Systems (1 mentions) Anti dap12, by Abcam (1 mentions) Anti p p38 thr180 tyr182, by Cell Signaling Technology (1 mentions) Anti v5, by Proteintech (1 mentions) Anti ikkα β, by Cell Signaling Technology (1 mentions) Anti rabbit igg hrp, by Santa Cruz Biotechnology (1 mentions)

5 protocols using amlexanox

1

TLR-Mediated Signaling Pathway Analysis

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Lipopolysaccharide (LPS, E. coli 0111: B4, TLR4 ligand), poly(I:C) (TLR3 ligand), and CpG ODN (TLR9 ligand) were purchased from Sigma-Aldrich. ChIP Grade Protein G Magnetic Beads, Cell Lysis Buffer, Anti-Myd88, Anti-Traf6, Anti-p65, Anti-p-p65 (Ser536), Anti-Ikkα/β, Anti-p-Ikkα/β (S176/180), Anti-IκBα, Anti-p-IκBα (S32), Anti-ERK, Anti-p-ERK (Thr202-Tyr204), Anti-JNK, Anti-p-JNK (Thr183-Tyr185), Anti-p38, Anti-p-p38 (Thr180-Tyr182) and Anti-Syk and Anti-p-Syk (Tyr-525/526) were from Cell Signaling Technology. Anti-DAP12 was purchased from Abcam. Anti-β-actin, Anti-rabbit IgG-HRP, and anti-mouse IgG-HRP were from Santa Cruz Biotechnology. Anti-LaminA/C, Anti-Flag and Anti-Myc and Anti-V5 were purchased from Proteintech. Anti-Ocilrp2 (AF3370) was from purchased R&D. The NF-κB inhibitor BAY11-7082 (10 μM), Tbk inhibitor Amlexanox (10 μM), Mek inhibitor PD98059 (10 μM), PI3K inhibitor Wortmannin (5 μM), Erk inhibitor SHC772984 (10 μM), Jnk inhibitor SP600125 (10 μM), or p38 inhibitor SB203580 (10 μM), Syk inhibitor R406 (5 μM) were from Selleckchem.
Cao M., Ma L., Yan C., Wang H., Ran M., Chen Y., Wang X., Liang X., Chai L, & Li X. (2022). Mouse Ocilrp2/Clec2i negatively regulates LPS-mediated IL-6 production by blocking Dap12-Syk interaction in macrophage. Frontiers in Immunology, 13, 984520.
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2

Colon Cancer Cell Lines and Inhibitor Assays

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Normal human colon epithelial CCD841 cells and human CRC cell lines, including HCT116, SW480, SW620, DLD-1, HT-29 and Lovo cells were obtained from the American Type Culture Collection (ATCC). The cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM; HyClone, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS; Gibco), 100 U/ml penicillin (M&C Gene Technology) and 100 μg/ml streptomycin (M&C Gene Technology) in a 37 ℃ incubator with a 5% CO2 and humidified atmosphere. The small molecular inhibitor including Amlexanox (S3648) was purchased from Selleck and the IKK-3 inhibitor (HY-14682) was purchased from MedChemExpress. A 50 mM stock solution of each inhibitor was prepared in dimethyl sulfoxide (DMSO; Sigma-Aldrich). Isopropy-β-D-thiogalactoside (IPTG) and neomycin were purchased from Sigma-Aldrich.
Liu G., Bao Y., Liu C., Zhu Q., Zhao L., Lu X., Zhu Q., Lv Y., Bai F., Wen H., Sun Y, & Zhu W.G. (2020). IKKε phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis. Theranostics, 10(5), 2358-2373.
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3

Affinity Purification and Immunoblotting Assay

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Inhibitors MLN120B (MedChemExpress, Monmouth Junction, NJ, USA, HY-15473), Pyridone 6 (MedChemExpress, HY-14435), and Amlexanox (Selleck Chemicals, Houston, TX, USA, S3648); MagStrep XT beads (IBA Lifesciences, Göttingen, NI, Germany, 2-4090-002) and buffer BXT (IBA Lifesciences, 2-1041-250); recombinant human IL-6 protein (PeproTech, Rocky Hill, NJ, USA, 200-06-5); mouse monoclonal antibodies against GAPDH (Proteintech, Wuhan, Hubei, China, 60004-1-Ig) and V5 (Thermo Fisher Scientific, MA5-15253); rabbit monoclonal antibodies against pY705-STAT3 (Cell Signaling Technology, Danvers, MA, USA, 9145T); rabbit polyclonal antibodies against STAT3 (Proteintech, 10253-2-AP), α-Tubulin (Proteintech, 11224-1-AP), Lamin B1 (Proteintech, 12987-1-AP), and strep-tag (GenScript Biotech, Piscataway, NJ, USA, A00626-40) were purchased from the indicated companies. Rabbit polyclonal antibodies against LCMV NP were raised against NP protein (aa1-558).
Wang Q., Xin Q., Shang W., Wan W., Xiao G, & Zhang L.K. (2021). Activation of the STAT3 Signaling Pathway by the RNA-Dependent RNA Polymerase Protein of Arenavirus. Viruses, 13(6), 976.
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4

Transfection and Inhibition Assays

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Cells were transfected with plasmids or siRNAs using Lipofectamine 3000 (Invitrogen) following the manufacturer’s protocol. Cells were plated in 24-well plates at a density of 1.5 x 105 cells/well and transfected with 200 ng plasmids per well for 24 h or 20 nM siRNA per well for 36 h. For co-transfection, cells were transfected with the indicated amounts of two plasmids for 24 h. The target sequences in mouse MOV10 for siRNAs were as follows: si1-MOV10: 5’-GGTACTAACCCTACGGCTT-3’; si2-MOV10: 5’-GCTATGAACTCCACATCTA-3’; si-IKKε: 5’-GGAGGCTGAATCACCAGAA-3’; si-MDA5: 5’- GCACUAUUCCAAGAACUAATT-3’. Amlexanox was purchased from Selleck (S3648). IFN alpha-IFNAR-IN-1 (HY-12836A), was purchased from MedChemExpress. Poly(I:C) and poly(dA:dT) were kind gifts from Chunfu Zheng at Fujian Medical University. Neuro-2a cells were treated with 1 μg/ml poly(I:C) for 16 h before analyzed by qRT-PCR. Poly(dA:dT) were transfected using Lipofectamine 3000 at a concentration of 1 μg/ml. For inhibition of transcription, actinomycin D (MedChemExpress, HY-17559) was used at a final concentration of 10 μg/ml.
Yang X., Xiang Z., Sun Z., Ji F., Ren K, & Pan D. (2022). Host MOV10 is induced to restrict herpes simplex virus 1 lytic infection by promoting type I interferon response. PLoS Pathogens, 18(2), e1010301.
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5

Immunoblot Analysis of Cellular Signaling

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The following primary antibodies were used for immunoblotting analysis: anti-p-P65 (#3033) and anti-p-IκBα (#2859) (Cell Signaling Technology), Anti-IL-1α-Fluorescein (#IC200F, R&D Systems), Anti-p16 (#10883-1-AP, Proteintech), anti-S100A13, IL-1α, p-P38, γ-H2AX, p-4EBP1, p-S6K1, RAS, E1A (Santa Cruz), anti-p21 and anti-β-tubulin (#BS1482M) (BioWorld). IgG (Santa Cruz, sc-2025). Human recombinant IL-1α (Sino Biological, 10128-HNCH).
DMSO (Sigma) was used as a solvent to dissolve TTM (Santa Cruz) and Amlexanox (Selleck). 4-OHT (Sigma) was dissolved in methanol. Doxorubicin (Sigma) was dissolved in sterile water.
Su Y., Xu C., Sun Z., Liang Y., Li G., Tong T, & Chen J. (2019). S100A13 promotes senescence-associated secretory phenotype and cellular senescence via modulation of non-classical secretion of IL-1α. Aging (Albany NY), 11(2), 549-572.
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