Circdhtkd1 probe

Manufactured by RiboBio

Sourced in United States

The CircDHTKD1 probe is a lab equipment product designed to detect the expression of the CircDHTKD1 gene. It functions as a molecular probe, enabling researchers to identify and quantify the presence of the CircDHTKD1 circular RNA transcript in biological samples.

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Lab products found in correlation

Oligo probe, by RiboBio (2 mentions) C 1 magnetic beads, by Thermo Fisher Scientific (2 mentions)

2 protocols using circdhtkd1 probe

Profiling circDHTKD1 and miRNA interactions

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C-1 magnetic beads (Life Technologies, USA) were pre-coated with circDHTKD1 probe or Oligo probe (RIBOBIO) at room temperature for 2 h. SCC9 and Cal27 cells transfected with vector or circDHTKD1 were lysed and incubated with the probe-coated magnetic beads at 4°C overnight and qRT-PCR was used for the expression analysis of circDHTKD1. In addition, cell lysates of SCC9 and Cal27 cells were also incubated with the above probe-coated magnetic beads followed by the enrichment detection of different miRNAs (miR-326, miR-330-5p, and miR-338-3p).

Wu Z., He X, & Chen S. (2021). Oncogenic circDHTKD1 promotes tumor growth and metastasis of oral squamous cell carcinoma in vitro and in vivo via upregulating miR-326-mediated GAB1. Brazilian Journal of Medical and Biological Research, 54(10), e10837.

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Analyzing CircRNA-miRNA Interactions

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C-1 magnetic beads (Life Technologies, Carlsbad, CA, USA) were conjugated with oligo probe or circDHTKD1 probe (RIBOBIO), followed by incubation with CaSki and SiHa cells at 4°C overnight. Then, miRNA levels were examined via RT-qPCR. For target binding between circ_0008460 and miR-197-3p, cells were transfected with biotin-coupled miR-197-3p mimic (Bio-miR-197-3p) or biotin-coupled mimic control (Bio-NC) and incubated with magnetic beads. The expression of circ_0008460 was measured via RT-qPCR.

Li S., Zhang H., Jiao Y., Song X., Wei L, & Liu X. (2022). Oxymatrine induces anti-tumor response in cervical cancer by modulating circ_0008460/miR-197-3p/ribonucleotide reductase subunit M2 (RRM2). Bioengineered, 13(5), 12912-12926.

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