Innotest htau antigen kit

CSF was obtained during routine diagnostic lumbar puncture according to a standardized protocol, in the outpatient clinic, from 09:30 to 10:30, after informed written consent had been obtained. CSF was collected in sterile polypropylene tubes and gently mixed to avoid gradient effects. Routine chemical measures were determined. The remaining CSF was centrifuged for 3 min at 3000 rpm, and aliquots were stored at −80 °C or in liquid nitrogen for subsequent dosages. CSF concentrations were measured in duplicate by an ELISA test (Innotest hTau antigen kit and Innotest phospho-tau 181P; Abeta42, Innogenetics, Ghent, Belgium). Inter-assay variability was less than 7%. According to our laboratory standards, the cut-off value was defined as Aβ_1–42 < 650 ng/L, total tau (t-Tau) > 400 ng/L and phosphorylated tau₁₈₁ (p-Tau₁₈₁) > 60 ng/L [38 (link)].

Lumbar puncture was performed in a subset of FTD patients according to a standardized protocol, in the outpatient clinic, at fasting, from 9.00 a.m. to 10.00 a.m, after informed written consent. CSF was collected in sterile polypropylene tubes and gently mixed to avoid gradient effects. Routine chemical measures were determined. CSF total-Tau, phospho-Tau and Abeta 42 concentrations were measured in duplicate by ELISA test (Innotest hTau Antigen kit and Innotest PHOSHO-TAU 181P, Innogenetics, Ghent, Belgium).
The remaining CSF was stored at −80 °C or in liquid nitrogen for subsequent anti-GluA3 antibody dosage. The same protocol used for serum was applied to test anti-GluA3 antibody peptide A, except for plates (Immulon 4HBX 96-well plates, Dynatech, Germany) and the working dilution (1:2 for CSF and 1:4500 for the secondary antibody).