Muscles were imaged with inverted Leica SP2 and SP8 (Leica Microsystems CMS, Mannheim, Germany) confocal microscopes equipped with HC PL APO 20× /0.75 IMM CORR, HC PL APO 63× /1.40 OIL CS2, and HC PL APO CS2 63× /1.2 W CORR objectives. Whole mount microscopy of hemidiaphragms used automated tile scan imaging with the Navigator module and automated stitching in the smooth mode with the Stitching module of Leica LAS-X 3.3.0 software suite. Projections of hemidiaphragms were rendered with the 3D Visualization module of Leica LAS-X 3.3.0 software suite.
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