SLBs were prepared
by spin-coating as described elsewhere.31 (link)A solution of 1 mg/mL total lipid concentration (either 100%
DOPC (Avanti Polar Lipids, USA) or 50% DOPC/50% cholesterol (Avanti
Polar Lipids)) both doped with DPPE-Abberior STAR Red (Abberior, Germany)
at a weight on weight ratio of 1:2000 in 1:2 methanol/chloroform was
spin-coated at 3200 rpm onto a clean 25 mm #1.5 glass coverslip (VWR,
UK). The resulting lipid film was hydrated with SLB buffer (150 mM
NaCl, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic
acid (HEPES) pH 7.4) and washed multiple times.
Glass coverslips
were cleaned using piranha edging (3:2 sulfuric
acid/hydrogen peroxide) for 2 h and not stored for longer than a week.
by spin-coating as described elsewhere.31 (link)A solution of 1 mg/mL total lipid concentration (either 100%
DOPC (Avanti Polar Lipids, USA) or 50% DOPC/50% cholesterol (Avanti
Polar Lipids)) both doped with DPPE-Abberior STAR Red (Abberior, Germany)
at a weight on weight ratio of 1:2000 in 1:2 methanol/chloroform was
spin-coated at 3200 rpm onto a clean 25 mm #1.5 glass coverslip (VWR,
UK). The resulting lipid film was hydrated with SLB buffer (150 mM
NaCl, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic
acid (HEPES) pH 7.4) and washed multiple times.
Glass coverslips
were cleaned using piranha edging (3:2 sulfuric
acid/hydrogen peroxide) for 2 h and not stored for longer than a week.