Osimertinib azd9291

Human EGFR-TKIs resistant NSCLC cell line NCI-H1975 (#No. CRL-5908TM) was purchased from ATCC (American type culture collection; Manassas, VA, USA). Immortalized human epithelial cell line BEAS-2B was also obtained from ATCC. Human lung squamous cell carcinoma and immortalized human liver cell line 95-D and HL7702, respectively, were purchased from Shanghai cell bank affiliated to the Chinese Academy of Sciences (Shanghai, China). H1975 and HL7702 cells were maintained in RPMI-1640 medium (Sigma, St. Louis, MO, USA) containing 10% fetal bovine serum (FBS, Gibco, USA). BEAS-2B and 95-D cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma, St. Louis, MO, USA) supplemented with 5 and 10% fetal bovine serum, respectively, in a humidified atmosphere containing 5% CO₂ at 37°C.
Osimertinib (AZD-9291), 10058-F4 (Myc-Max disruptor), and STF-31 (a specific Glut-1 inhibitor) were purchased from MedChem Express (Monmouth Junction, NJ, USA). KC7F2, gefitinib, and cisplatin were obtained from APExBIO (Houston, TX, USA). Chloroquine (CQ) was acquired from Sigma (St. Louis, MO, USA). Rapamycin was obtained from Selleck Chemicals (Houston, TX, USA). Cell Counting Kit-8 (CCK-8) was purchased from Beyotime Biotech (Shanghai, China).