Elisas

Irisin in mouse serum was measured using commercially-available ELISAs (Phoenix Pharmaceuticals, Burlingame, CA, USA).

The study protocol was approved by the Institutional Review Board of Shaare Zedek Medical Center. Written informed consent was obtained from each of the participants. Subjects with genetically confirmed PWS were recruited from the Israeli National Multidisciplinary PWS Clinic at Shaare Zedek Medical Center, Jerusalem. The control group was comprised of volunteers: 9 with body mass index (BMI) <25 kg/m² (6 males/3 females) and 24 overweight or obese (11 males/13 females) with BMI >25 kg/m². Subjects with chronic illnesses, uncontrolled hypothyroidism, diabetes mellitus, or exposure to hormone medication including estrogen-containing compounds, glucocorticoids, or androgens were excluded. Venous whole blood was collected from subjects, separated to plasma, and stored at −80 °C until further assessed. The Israeli participants underwent biochemical testing, following a 12 h fast for serum lipid profile, glucose and insulin. Measurements of leptin and adiponectin were performed by ELISAs (Phoenix, Pharmaceuticals, USA). The homeostasis model assessment insulin resistance (HOMA-IR) was calculated as fasting serum insulin ([μU/mL] X fasting plasma glucose [mmol/L]/22.5). Height and weight were measured and the BMI was calculated.