Anti flag m2 agarose beads a2220

Manufactured by Merck Group

Anti-Flag M2 agarose beads (A2220) are affinity chromatography resin particles used for the purification of proteins fused with the Flag peptide tag. The beads are composed of cross-linked agarose and contain the Anti-Flag M2 monoclonal antibody that recognizes the Flag tag sequence.

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Lab products found in correlation

Anti ha agarose beads a2095, by Merck Group (1 mentions) Anti flag antibody f7425, by Merck Group (1 mentions) Igg sepharose beads, by GE Healthcare (1 mentions) Mab3118, by Merck Group (1 mentions) Ab15083, by Abcam (1 mentions)

Spelling variants of this product

Anti-FLAG M2 (1022 citations) FLAG M2 (383 citations) Anti-FLAG beads (153 citations) Anti-Flag agarose (78 citations) Flag M2 agarose (56 citations) Anti-Flag (A2220, (8 citations) Anti-FLAG beads (A2220) (5 citations) Anti-FLAG-agarose (A2220; (3 citations) Anti-Flag M2 beads (A2220) (2 citations) Anti-Flag M2 (A2220, (2 citations)

2 protocols using anti flag m2 agarose beads a2220

Analyzing DNA Damage Response

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Anti-Flag M2 agarose beads (A2220) and anti-HA agarose beads (A2095) were purchased from Sigma. Anti-Chk1 (sc-56288) and anti-p53 (sc-55476) antibodies were purchased from Santa Cruz. Anti-PARP-1 (9532), anti-pChk1S345 (2341), anti-pChk1S317 (2344), anti-p-p53 (9284), anti-γ-H2AX (9718), anti-pATR-S428 (2853), anti-pATM-S1981 (13050) and anti-pCdc25C (9528S) antibodies were purchased from Cell Signaling Technologies. Cycloheximide (C4859), cisplatin (479306), and imidazole (12399) were purchased from Sigma. Ni-NTA resin (635659) was purchased from Clontech.

Moses N., Zhang M., Wu J.Y., Hu C., Xiang S., Geng X., Chen Y., Bai W., Zhang Y.W., Bepler G, & Zhang X.M. (2020). HDAC6 Regulates Radiosensitivity of Non-Small Cell Lung Cancer by Promoting Degradation of Chk1. Cells, 9(10), 2237.

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Protein Interaction Assays Using Antibodies

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Anti-FLAG antibody (F7425) and anti-FLAG M2 agarose beads (A2220) were purchased from Sigma. Anti-Myc tag and anti-HA tag antibodies used for ChIP and IP were from Millipore (05–724 and 05-904 respectively). Anti-myc, anti-HA and anti-FLAG antibodies used in western probings were from Santa Cruz ((9E10) SC-40 and (Y-11) SC-805) and Millipore (MAB3118) respectively. IgG sepharose beads were from GE Healthcare. Anti-H3 antibody (abcam 1791) and antibody against phosphorylated H2A S129 (ab15083) were from Abcam.

Bhalla P., Shukla A., Vernekar D.V., Arimbasseri A.G., Sandhu K.S, & Bhargava P. (2019). Yeast PAF1 complex counters the pol III accumulation and replication stress on the tRNA genes. Scientific Reports, 9, 12892.

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