Supersignal west pico plus chemiluminescent substrate 34577

Human Aortic Endothelial Cells (CC-2535), EGM-2 BulletKit (CC-3162) and EBM-2 (00190860) were obtained from Lonza (Walkersville, MD, USA). hrPDGF-C (SRP3139) and Valinomycin (V0627) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Mitotracker Green FM (M7514), Hoechst 34580 (H21486) N,N-dimethyl-4-[5-(4-methyl-1-piperazinyl)[2,5′-bi-1H-benzimidazol]-2′yl] and SuperSignal^TM west pico PLUS chemiluminescent substrate (34577) were obtained from Thermo Fisher Scientific/Invitrogen (Chelmsford, MA, USA). Protease and phosphatase inhibitor cocktail (5872), antibodies against OPA1 (D7C1A), MFN1 (D6E2S), MFN2 (D1E9), DRP1 (D6C7), DRP1^pS616, β actin (8H10D10), Anti-rabbit IgG HRP-linked and anti-mouse IgG HRP-linked were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibody against (ab96764) were obtained from Abcam (San Francisco, CA, USA).

Human aortic endothelial cells (CC-2535), EGM-2 BulletKit (CC-3162) and EBM-2 (00190860) were obtained from Lonza (Walkersville, MD, USA). hrPDGF-C (SRP3139), Rotenone (R8875), and MitoTEMPO (SML0737) were obtained from Sigma-Aldrich (St. Louis, MO, USA). CM-H₂DCFDA 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate, acetyl ester (C6827), MitoSOX^TM (M36008), anti-rabbit IgG Alexa Fluor 488 (A11008), SuperSignal^TM West Pico PLUS chemiluminescent substrate (34577), TRIzol^TM reagent (15596-026), and M-MLV reverse transcriptase (28025013) were obtained from ThermoFisher Scientific/Invitrogen (Chelmsford, MA, USA). Oligo (dt)18 primer (38029) and DNTPs mix (39029) were obtained from Meridian Life Sciences (Memphis, TN, USA). FastStar essential DNA Green Master (06402712001) was obtained from Applied Biosystems (Waltham, CA, USA). Quantikine ELISA human PDGF-CC (DC00) was obtained from R & D Systems (Minneapolis, MN, USA). A Superoxide Dismutase Activity Assay Kit was obtained from Abcam (ab65354) (Cambridge, UK). Antibodies against PDGFRα (D1E1E), PDGFRβ (28E1), SOD2 (D3X8F XP^®), catalase (D4P7B XP^®), GPx1, β actin (8H10D10), Anti-rabbit IgG HRP-linked, and anti-mouse IgG HRP-linked were obtained from Cell Signaling Technology (Danvers, MA, USA).