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Human phospho vegfr3 elisa kit

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The Human phospho-VEGFR3 Elisa Kit is a quantitative sandwich enzyme-linked immunosorbent assay designed to measure the levels of phosphorylated VEGFR3 in human cell and tissue lysates.

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Lab products found in correlation

Substrate solution, by R&D Systems (2 mentions) Streptavidin hrp, by Vector Laboratories (1 mentions)

2 protocols using human phospho vegfr3 elisa kit

1

VEGFR-3 Phosphorylation Assay

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Serum-starved cells were treated with VEGF-C species (1 µg/mL) and assayed using a human phospho-VEGFR3 Elisa Kit (R&D). Treated cells were lysed (R&D lysis buffer; 30 minutes, 4°C), spun-down, diluted, added to a precoated anti–VEGFR-3 plate overnight (4°C), followed by antiphosphotyrosine horseradish peroxidase (included in Kit). Biotin anti–VEGFR-3 (Reliatech) labeled with streptavidin-horseradish peroxidase (Vector) was added to detect total VEGFR3. After incubating in substrate solution (R&D), reactions were stopped with 2N sulfuric acid. Plates were assayed at absorbance A450 nm, with values corrected against total VEGFR-3.
Johns S.C., Yin X., Jeltsch M., Bishop J.R., Schuksz M., El Ghazal R., Wilcox-Adelman S.A., Alitalo K, & Fuster M.M. (2016). Functional Importance of a Proteoglycan Coreceptor in Pathologic Lymphangiogenesis. Circulation Research, 119(2), 210-221.
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2

Quantifying VEGFR3 Phosphorylation

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Serum-starved cells were treated with VEGF-C species (1μg/ml), and assayed using a human phospho-VEGFR3 Elisa Kit (R&D). Treated cells were lysed (R&D lysis buffer; 30min, 4°C), spun-down, diluted, added to a pre-coated anti-VEGFR3 plate overnight (4°C), followed by anti-phosphotyrosine-HRP (included in Kit). Biotin-anti-VEGFR3 (Reliatech) labeled with streptavidin-HRP (Vector) was added to detect total VEGFR3. After incubating in substrate solution (R&D), reactions were stopped with 2N sulfuric acid. Plates were assayed at absorbance A450nm, with values corrected against total VEGFR-3.
Johns S.C., Yin X., Jeltsch M., Bishop J.R., Schuksz M., El Ghazal R., Wilcox-Adelman S.A., Alitalo K, & Fuster M.M. (2016). Functional Importance of a Proteoglycan Coreceptor in Pathologic Lymphangiogenesis. Circulation Research, 119(2), 210-221.
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