Dorsal skins were frozen in OCT, sectioned at 6 μm and used for Hematoxylin and Eosin staining or for immunofluorescence. Sections were fixed in 4% formaldehyde and subjected to indirect immunofluorescence. When staining with mouse mAbs, the MOM Fluorescent Kit (Vector) was used. Primary antibodies used were: mouse monoclonal Mi-2β [16G4]10 (link), rabbit polyclonal K5 (BAbCo), rabbit polyclonal K1 (BAbCo), rabbit polyclonal Loricrin (BAbCo), rabbit polyclonal K6 (BAbCo), goat polyclonal TSLP (R&D), rat monoclonal Ki67 (BD Biosciences, SolA15). Fluorescence-conjugated secondary antibodies for primary antibodies developed in rabbit, rat, or goat were obtained from Jackson ImmunoResearch Laboratories. For BrdU incorporation analysis, mice were injected i.p. with BrdU (100 μg/g body weight) (Molecular Probes). 2 h later, dorsal skin was harvested and stained with BrdU-specific antibody conjugated with Alexa Fluor 546 (Molecular Probes). DAPI was used to stain nuclei. Images were taken with the Olympus BX50 or Nikon A1 Confocal microscope.
Goat polyclonal tslp
Manufactured by R&D Systems
Goat polyclonal TSLP is a laboratory reagent used for research purposes. It is a purified antibody raised in goats against the Thymic Stromal Lymphopoietin (TSLP) protein. This antibody can be used to detect and quantify TSLP expression in various experimental systems.
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2 protocols using goat polyclonal tslp
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Immunofluorescence Analysis of Murine Skin
2
Immunofluorescence Analysis of Murine Skin
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Dorsal skins were frozen in OCT, sectioned at 6 μm and used for Hematoxylin and Eosin staining or for immunofluorescence. Sections were fixed in 4% formaldehyde and subjected to indirect immunofluorescence. When staining with mouse mAbs, the MOM Fluorescent Kit (Vector) was used. Primary antibodies used were: mouse monoclonal Mi-2β [16G4]10 (link), rabbit polyclonal K5 (BAbCo), rabbit polyclonal K1 (BAbCo), rabbit polyclonal Loricrin (BAbCo), rabbit polyclonal K6 (BAbCo), goat polyclonal TSLP (R&D), rat monoclonal Ki67 (BD Biosciences, SolA15). Fluorescence-conjugated secondary antibodies for primary antibodies developed in rabbit, rat, or goat were obtained from Jackson ImmunoResearch Laboratories. For BrdU incorporation analysis, mice were injected i.p. with BrdU (100 μg/g body weight) (Molecular Probes). 2 h later, dorsal skin was harvested and stained with BrdU-specific antibody conjugated with Alexa Fluor 546 (Molecular Probes). DAPI was used to stain nuclei. Images were taken with the Olympus BX50 or Nikon A1 Confocal microscope.
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