RNeasy Plus Micro kit (Qiagen, #74034) was used to isolate RNA from mPFC (infralimbic/prelimbic) punches. A cDNA reverse transcription kit containing RNA inhibitor (Applied Biosystems, #4374966) was used to convert 200ng of RNA to single-stranded cDNA, following the instructions of the manufacturer. Real-time PCR reactions were performed in triplicate via TaqMan primer and probe sets from Applied Biosystems (c-fos, Mm00487425_m1; Arc, Mm01204954_g1; Gapdh, Mm99999915_g1; Tubulin, Mm00495806_g1). The following run parameters on an ABI StepOne machine were used: 50°C for 2 mins, 95°C for 20 secs, 40 cycles of 95°C for 1 sec, 60°C for 20 sec. Each gene’s relative expression was determined using the −2(ΔΔCt) method. Levels were normalized to those of Tubulin, which did not itself differ between groups. Relative changes were normalized to the GFP no object control group.
C fos
Manufactured by Thermo Fisher Scientific
Sourced in United States
C-fos is a gene that encodes for a transcription factor protein involved in cellular processes. It is commonly used as a marker for cellular activity and activation in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Taqman primer and probe set, by Thermo Fisher Scientific (1 mentions)
Rneasy plus micro kit, by Qiagen (1 mentions)
18s rrna, by Thermo Fisher Scientific (1 mentions)
Mmp 9, by Thermo Fisher Scientific (1 mentions)
Taqman universal master mix 2, by Thermo Fisher Scientific (1 mentions)
Prdm1, by Thermo Fisher Scientific (1 mentions)
Fast red violet lb salt, by Merck Group (1 mentions)
Hplc grade solvents, by Thermo Fisher Scientific (1 mentions)
Formic acid, by Samchun (1 mentions)
2 protocols using c fos
1
Quantifying Neuronal Gene Expression in mPFC
2
Osteoclast Differentiation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Formic acid was acquired from Samchun (Pyeongtaek, Republic of Korea). All HPLC-grade solvents and fetal bovine serum (FBS) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Minimum Essential Medium, Alpha Modification (α-MEM), was purchased from Hyclone (Logan, UT, USA). AS-MX phosphate, fast red violet LB salt, and naphthol were procured from Sigma-Aldrich (St. Louis, MO, USA). Antibodies targeting c-Fos and NFATc1 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies targeting TRAF6, c-Src, CAII and HRP-conjugated secondary antibodies (anti-rabbit IgG and anti-mouse IgG) were purchased from Cell Signaling Technology (Danvers, MA, USA). TaqMan Universal Master Mix II and TaqMan probes for the target genes, including c-Fos, NFATc1, Prdm1, Irf8, MafB, Ctsk, Itgβ3, Mmp9, and 18S rRNA, were obtained from Applied Biosystems (Foster City, CA, USA). TSN standard was purchased from ChemFace (Wuhan, China).
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