Naoh granules

In this study, fungi provided by the RikenBRC (Kyoto, Japan) through the National BioResource Project of the MEXT/AMED Japan (Botryosphaeria dothidea JCM 2738 (BD), Fusarium oxysporum f.sp. langenariae JCM 9293 (FO), and Fusarium verticillioides JCM 23107 (FV)) from the culture collection of the Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology (Zagreb, Croatia) (Mucor indicus DSM 2185 (MI)) and the CBS Fungal Diversity Centre and Technology (Utrecht, The Netherlands) (Paecilomyces variotii CBS 372.70 (PV), Myceliophthora thermophila CBS 663.74 (MT), and Thielavia terrestris CBS 456.75 (TT)), were used as working microorganisms. Cultures were maintained on standard potato dextrose agar (Difco, BD, Franklin Lakes, NJ, USA).
The media composition used for the cultivations of fungi are given in Table 1. All chemicals used in media preperation were acquired from Sigma Aldrich, St. Louis, MO, USA, if not stated otherwise. The pH value of the media was set to a value of 6 with the addition of NaOH granules (Sigma Aldrich, St. Louis, MO, USA), and afterwards, the media were sterilized at 121 °C for 15 min. The salt solution was filtered through a disposable sterile syringe filter (CHROMAFIL Xtra PA-20/25, Duren, Germany) and added to the media before use (Table 1).