Water quality (TDS, DO, conductivity, pH, and temperature) was also measured at each site using a YSI Pro Plus probe. Anions, cations, and organic acids in water and sediment pore water were measured using a Dionex ICS 5000+ instrument (Thermo Fisher Scientific, Waltham, MA, USA) as described in the supplemental material. A list of all ions can be found in Table S10. Dissolved trace elements were measured at the University of Tennessee Water Chemistry Core Facility using an inductively coupled plasma optical emission spectrometer (ICP-OES) iCAP 7400 system (Thermo Fisher Scientific) following EPA method 200.7 rev 4.4 (59 ). As an internal check, Ca, K, and Na were measured using both IC and ICPOES; samples measured with ICP are labeled X_ICP. The Wilcoxon rank sum test was used to determine statistical significance (α = 0.05) based on UOG status.
Dionex ics 5000 instrument
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Dionex ICS 5000+ instrument is a high-performance ion chromatography system designed for the analysis of ionic species. It is capable of performing ion exchange, ion exclusion, and ion pair chromatography. The instrument features intelligent automation, enhanced detection capabilities, and advanced software for comprehensive data analysis.
Automatically generated - may contain errors
Lab products found in correlation
Icap 7400 system, by Thermo Fisher Scientific (2 mentions)
8453 spectrometer, by Agilent Technologies (1 mentions)
Synergy plate reader, by Agilent Technologies (1 mentions)
Autoflex mass spectrometer, by Bruker (1 mentions)
Sx 20 instrument, by Applied Photophysics (1 mentions)
Emxplus spectrometer, by Bruker (1 mentions)
3 protocols using dionex ics 5000 instrument
1
Water Quality and Geochemical Analysis
2
Purification and Characterization of LPMOAz
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LPMOAz and its variants were constructed in a pET-9b vector and expressed in BL21-DE3 Escherichia coli strain. Ion exchange and size exclusion chromatography were performed to purify the proteins. UV–Vis spectroscopy results were acquired on a Cary 60 spectrometer for metal titration or an Agilent 8453 spectrometer for monitoring the reactions with oxidants. Stopped-flow experiments were performed on an Applied Photophysics SX-20 instrument. CW-EPR results were acquired on a Bruker EMX Plus spectrometer. Pulse-EPR results were acquired on a Bruker ELEXSYS E-580-10 FT-EPR spectrometer. Simulation of the EPR data was performed using the Easyspin toolbox (100 (link)). Activity assays with 4-NPGP, cellulose, and starch were performed with reconstituted CuZn–LPMOAz supplied with sodium or ammonium ascorbate in aerobic environment. The 4-NPGP assays were monitored on a BioTek Synergy plate reader. MALDI-TOF MS spectra were acquired on a Bruker Autoflex mass spectrometer. HPAEC analyses were performed on a Thermo Fisher Scientific Dionex ICS-5000 instrument.
Detailed procedures of the experiments are available inSI Appendix .
Detailed procedures of the experiments are available in
3
Water Quality and Geochemical Analysis
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Water quality (TDS, DO, conductivity, pH, and temperature) was also measured at each site using a YSI Pro Plus probe. Anions, cations, and organic acids in water and sediment pore water were measured using a Dionex ICS 5000+ instrument (Thermo Fisher Scientific, Waltham, MA, USA) as described in the supplemental material. A list of all ions can be found in Table S10. Dissolved trace elements were measured at the University of Tennessee Water Chemistry Core Facility using an inductively coupled plasma optical emission spectrometer (ICP-OES) iCAP 7400 system (Thermo Fisher Scientific) following EPA method 200.7 rev 4.4 (59 ). As an internal check, Ca, K, and Na were measured using both IC and ICPOES; samples measured with ICP are labeled X_ICP. The Wilcoxon rank sum test was used to determine statistical significance (α = 0.05) based on UOG status.
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