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2 protocols using l histidine

1

Comprehensive Biochemical Analysis Protocol

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SCAAs (Cys, CySS, GSH, and HCys), 1,4-benzoquinone (pBQ), 3-mercaptopropionic acid (3-MPA), PR, BPR, PV, and other amino acids [L–alanine (Ala), L–arginine (Arg), L–aspartic acid (Asp), L–glutamine (Gln), L–glutamic acid (Gln), glycine (Gly), L–histidine (His), L–isoleucine (Ile), L–leucine (Leu), L–lysine (Lys), L–methionine (Met), L–phenylalanine (Phe), L–proline (Pro), L–serine (Ser), L–threonine (Thr), L–tryptophan (Trp), L–tyrosine (Tyr), and L–valine (Val)] were purchased from Tokyo Chemical Industries Co. Inc. (Tokyo, Japan). Additionally, ARS, sodium chloride, methanol, and zinc nitrate hexahydrate were purchased from FUJIFILM Wako Pure Chemical Co. Inc. (Osaka, Japan). N-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) was purchased from Dojindo Laboratories (Kumamoto, Japan). All chemicals were used without further purification. All aqueous samples were prepared with Milli-Q water (18.2 MΩcm) (Millipore, Bedford, MA, United States).
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2

Fluorescent Labeling of Trastuzumab and Amino Acids

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Herceptin® (trastuzumab), a Food and Drug Administration-approved humanized anti-HER2 IgG1 monoclonal antibody, was obtained from Roche Pharma (Basel, Switzerland). Amine-reactive ATTO655-N-hydroxysuccinimidyl ester (ATTO655-NHS ester; λexem: 663/684), ATTO680-NHS esterexem: 680/700), ATTO700-NHS ester (λexem: 700/719), and free ATTO680-COOH were purchased from ATTO TEC (Siegen, Germany). NHS-Fluoresceinexem: 494/518), Alexa Fluor® 488-NHS ester (λexem: 494/518), and Alexa Fluor® 680-NHS ester (λexem: 679/702) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). L-Trptophan and L-methionine were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-Acetyl-L-Tyrosine and L-histidine were purchased from Tokyo Chemical Industry (Tokyo, Japan).
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