Collagen gels were prepared by mixing fibroblasts with PureCol bovine type I collagen (Advanced Biomatrix, San Diego, California, USA), 5x Dulbecco's modified Eagle's medium (DMEM), 0.1 M NaOH and distilled water (final concentrations: 1.7 mg/mL PureCol, 1x DMEM and 3 mM NaOH) in the presence of 100 nM 1,25(OH)2D3 or vehicle. The mixture was seeded in 24-well cell culture plates and allowed to polymerise. Then, culture medium with 100 nM 1,25(OH)2D3 or vehicle was added. After 24 h and to initiate gel contraction (time 0), gels were released from the 24-well plates and transferred into 6-well plates containing culture medium with 100 nM 1,25(OH)2D3 or vehicle. At the indicated times, images were taken with an E4500 digital camera (Nikon, Tokyo, Japan) mounted in a MZ6 modular stereomicroscope (Leica, Wetzlar, Germany), and gel area was measured with ImageJ (National Institutes of Health, Bethesda, Maryland, USA). Images were processed using Adobe Photoshop CS6 (San Jose, California, USA). Experiments were performed using triplicates.
Mz6 modular stereomicroscope
Manufactured by Leica
Sourced in Japan
The Leica MZ6 modular stereomicroscope is a versatile optical instrument designed for a range of applications. It features binocular observation, providing a three-dimensional view of the specimen. The MZ6 offers a magnification range and optical performance suitable for various tasks, though its specific intended use is not detailed here.
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Lab products found in correlation
2 protocols using mz6 modular stereomicroscope
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Collagen Gel Contraction Assay
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Collagen Gel Contraction Assay
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Assays were performed as described previously [27 (link)], preparing the collagen gels by mixing fibroblasts with PureCol bovine type I collagen (Advanced Biomatrix, San Diego, CA, USA), 5 × DMEM, 0.1 M NaOH and distilled water (final concentrations: 1.7 mg/mL PureCol, 1 × DMEM and 3 mMNaOH). The mixture was seeded in 24-well cell culture plates and allowed to polymerize. After 24 h, and to initiate gel contraction (time 0), gels were released from the 24-well plates and transferred to 6-well plates containing culture medium with raloxifene (5 μM), NAC (1 mM), losartan (10 μM) or the vehicle alone. At the times indicated, images were taken with an E4500 digital camera (Nikon, Tokyo, Japan) mounted on a MZ6 modular stereomicroscope (Leica, Wetzlar, Germany), and the gel area was measured with ImageJ (National Institutes of Health, Bethesda, MD, USA). Images were processed using Adobe Photoshop CS6 (San Jose, CA, USA) and the experiments were performed in triplicate.
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