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3 protocols using lauric acid

1

Fatty Acid and Lipid Standards

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Caprylic acid (C8:0), capric acid (C10:0), lauric acid (C12:0), myristic acid (C14:0), stearic acid (C18:0), arachidonic acid (C20:4n6), and methyl-d3 stearate standard were obtained from Wako Pure Chemicals (Tokyo, Japan).
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2

Antimicrobial Activity of Fatty Acids

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The molecular structures of the materials evaluated in the present study are shown in Fig. 1. Myristic acid (C14:0 fatty acid, ≥ 99%) was purchased from Sigma-Aldrich Co. LLC (St. Louis, USA) . Lactic acid, lauric acid (C12:0 fatty acid, ≥ 99%) , palmitoleic acid (C16:1 fatty acid, ≥ 99%) and 0.1 mol L -1 phosphate buffer solution (pH 6) were obtained from Fujifilm Wako Pure Chemical Industries Ltd. (Osaka, Japan) . Ethanol, sodium chloride, beef extract, and polypeptone were purchased from Junsei Chemical Co. (Tokyo, Japan) , Kanto Chemical Co., Inc. (Tokyo, Japan) , Nacalai Tesque Inc. (Kyoto, Japan) , and Nihon Pharmaceutical Co., Ltd. (Tokyo, Japan) , respectively. Agar powder was purchased from Nissui Pharmaceutical Co., Ltd. (Tokyo, Japan) . S. aureus (NBRC13276) and S. epidermidis (NBRC12993) were obtained from the National Institute of Technology and Evaluation (Tokyo, Japan) . Water was purified using a Demi-Ace Model DX-15 demineralizer (Kurita Water Industries Ltd., Tokyo, Japan) .
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3

Synthesis and Characterization of PVA Films

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(weight-average molecular weights (M w ) = 150,000), trifluoroacetic anhydride (TFAA), acetic acid, propionic acid, butyric acid, valeric acid, hexanoic acid, octanoic acid, decanoic acid, lauric acid, ethanol, and chloroform were purchased from Wako Pure Chemicals (Tokyo, Japan). PVA films were provided by Kuraray. All reagents are used without further purification.
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