Tcf1 2203 s

Manufactured by Cell Signaling Technology

Sourced in United States

TCF1 (#2203 S) is a primary antibody that specifically recognizes the Transcription Factor 1 (TCF1) protein. TCF1 is a key transcription factor involved in the Wnt signaling pathway.

Automatically generated - may contain errors

Lab products found in correlation

Sc 47778 hrp, by Santa Cruz Biotechnology (1 mentions) Cd45ra pe cy7, by BioLegend (1 mentions) Dusp6 ab76310, by Abcam (1 mentions) Sb216763 s3442, by Merck Group (1 mentions) Cd69 percp cy5.5 560738, by BioLegend (1 mentions) Cd62l pe 304806, by BioLegend (1 mentions) Electrochemiluminescence kit, by Cytiva (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) β actin sc 8432, by Santa Cruz Biotechnology (1 mentions)

2 protocols using tcf1 2203 s

Immunoblotting and ChIP-PCR Assays

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Antibodies specific for TCF1 (#2203 S) and pERK (T202/Y204, #4377 S) from Cell Signaling Technology, and DUSP6 (ab76310) from Abcam, were used at a dilution of 1 in 1000 for immunoblotting or 1 in 50 for ChIP-PCR. Antibodies specific for β-actin were from Santa Cruz Biotechnology (sc-47778 HRP) and used at 1 in 10000. Fluorochrome-conjugated antibodies were from BD Biosciences including CD4-APC (555349), CD69- PerCP-Cy5.5 (560738) and CD45RA-PE-Cy™7 and BioLegend including CD62L-PE (304806), CD25-APC (302610) and human IL-2 Alexa-Fluor-488 (500314). All antibodies for flow cytometry were used at 1 in 50. PIM1 inhibitor (526521), GSK3β inhibitors, BIO (2’Z,3’E)-6-Bromoindirubin-3ʹ-oxime (361550) and SB216763 (S3442), were from Sigma.

Ye Z., Gould T.M., Zhang H., Jin J., Weyand C.M, & Goronzy J.J. (2021). The GSK3β-β-catenin-TCF1 pathway improves naive T cell activation in old adults by upregulating miR-181a. NPJ Aging and Mechanisms of Disease, 7, 4.

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Western Blot Analysis of Transcription Factors

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Antibodies against Blimp1 (9115 S, encoding by Prdm1) and TCF-1 (2203S, encoding by Tcf7) were purchased from Cell Signaling (Beverly, MA, USA). Antibodies against Smad4 (sc-7966) and β-actin (sc-8432) were obtained from Santa Cruz. Antibodies against Bcl6 (648301) and T-bet (644801, encoding by Tbx21) were obtained from BioLegend. Total protein extracts were prepared and dissolved in SDS sample buffer. Protein extracts were separated on 10% SDS-PAGE gels and transferred to polyvinylidene difluoride membranes (Millipore, Billerica, MA, USA). The membranes were probed with antibodies and visualized with an electrochemiluminescence kit (Amersham, Uppsala, Sweden).

Cao J., Zhang X., Wang Q., Qiu G., Hou C., Wang J., Cheng Q., Lan Y., Han H., Shen H., Zhang Y., Yang X., Shen B, & Zhang J. (2015). Smad4 represses the generation of memory-precursor effector T cells but is required for the differentiation of central memory T cells. Cell Death & Disease, 6(11), e1984-.

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