Diuron was purchased from Sigma Aldrich (Barcelona, Spain). Malt extract was acquired from Scharlau (Barcelona, Spain). High-performance liquid chromatography (HPLC) grade acetonitrile was supplied by Merck (Darmstadt, Germany). All chemical used were of high purity grade.
Malt extract
Manufactured by Scharlab
Sourced in Spain
Malt extract is a viscous, sweet liquid produced by the partial hydrolysis of starch from malted cereal grains, typically barley. It serves as a nutrient source for microbial growth in various laboratory applications.
Automatically generated - may contain errors
3 protocols using malt extract
1
Diuron Extraction and Analysis
2
Fluorescent Probes and Fungal Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
5,50,6,60-Tetrachloro-1,10,3,300-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1, 3520-43-2, MW 65223), propidium iodide (PI, 25535-16-4, MW 66839), calcein-AM (148504-34-1, MW 99486), and Hoechst 33342 (23491-52-3, MW 61599) were from Invitrogen (Carlsbad, CA, USA). The purification of ophiobolin A, as well as stephacidin B and avrainvillamide, is described in [16 (link)] and [14 (link)], respectively. Alamethicin A4665 (Trichoderma arundinaceum) (27061-78-5, a mixture of alamethicin F50 peptaibols, MW 1962, 1976, 1976, and 1990), sterigmatocystin, and ochratoxin A were obtained from the Sigma-Aldrich Corporation (St. Louis, MO, USA). Malt extract and tryptic soy agar were from Scharlab (Barcelona, Spain). The other chemicals were of an analytical grade and purchased from local suppliers.
3
Isolation and Cultivation of Pleurotus pulmonarius Mycelium
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The inner portion of the basidiomata of P. pulmonarius, an oyster mushroom, was excised by a flamed scalpel blade under sterile conditions and placed onto on malt extract agar medium (MEA) (Scharlab, Barcelona, Spain) (Fig. S2A). Seven-day-old mycelium grown in the malt yeast peptone (MYP) broth [7 g/L malt extract (Scharlab), 1 g/L peptone (Amresco, Solon, Cleveland, USA) and 0.5 g/L yeast extract (ACE Biolabs, Amsterdam, Netherlands)] was harvested and homogenized with tissue grinders (Kimble Chase, Vineland, NJ, USA). The mycelium powder (Fig. S2B) was stored at 4°C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!