The concentration of thiobarbituric acid reactive substances (TBARs) was used as marker of oxidative stress in kidney, liver, and serum. Tissues were homogenized in a radioimmunoprecipitation (RIPA) buffer (Sigma Aldrich, Cat No. R-0278) in a ratio of 1:10 w/v and then centrifuged at 1600× g and 4 °C for 10 min. The measurement was performed on the serum and supernatant of tissues using the thiobarbituric acid reactive substances (TBARs) assay kit No. 10009055 (Cayman Chemical Company, Ann Arbor, MI, USA) in accordance with the manufacturer’s instructions.
Tbars assay kit no 10009055
Manufactured by Cayman Chemical
Sourced in United States
The TBARs Assay Kit No. 10009055 is a quantitative colorimetric assay designed to measure the levels of thiobarbituric acid reactive substances (TBARs) in a variety of sample types. The kit provides the necessary reagents and protocols to perform the assay.
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2 protocols using tbars assay kit no 10009055
1
Oxidative Stress Quantification in Tissues
2
Oxidative Stress Assessment in Rat Tissues
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Oxidative stress in rat heart and liver was assessed by measuring MDA, which is the most abundant end-product of lipid peroxidation. Tissues were homogenised in a radioimmunoprecipitation (RIPA) buffer (Sigma Aldrich, Cat No. R-0278) in a ratio of 1:10 w/v and then centrifuged at 1600xg and 4 °C for 10 min. The measurement was performed on the supernatant using the thiobarbituric acid reactive substances (TBARS) assay kit No. 10009055 (Cayman Chemical Company, Ann Arbor, Michigan. USA) according to the instructions provided by the manufacturer.
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