Trichloroacetic acid tca

Manufactured by Biopack

Sourced in Argentina

Trichloroacetic acid (TCA) is a chemical compound commonly used in laboratory settings. It functions as a protein precipitation agent, facilitating the separation and analysis of proteins from complex biological samples.

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Trichloroacetic Acid (TCA) pro analysis grade

4 protocols using trichloroacetic acid tca

Murine AChE and Human BChE Activity Assays

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All chemicals and drugs – DPPH, 6-hydroxy-2,5,7,8-tetramethylchromium-2-carboxylic acid (Trolox), ABTS, ferrozine, EDTA, 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), acetylthiocholine iodide and butyrylthiocholine iodide, reduced glutathione, gallic acid, tacrine, pargyline, clorgyline, isatin, L-deprenyl were purchased from Sigma-Aldrich®, Argentina. TBA, trichloroacetic acid (TCA) and butylated hydroxytoluene (BHT) were purchased from Biopack® Productos Químicos, Argentina. Vitamin E was purchased from Casasco Laboratories (Argentina).
Murine mAChE and recombinant hBChE were kindly provided by Xavier Brazzolotto and Florian Nachon (IRBA, Brétigny-sur-Orge, France). Recombinant human microsomal hMAO (expressed in baculovirus-infected insect cells (BTI-TN-5B1-4 cells)), horseradish peroxidase (type II, lyophilized powder) and p-tyramine hydrochloride were obtained from Sigma Aldrich (Sigma Aldrich, St. Louis, MO, USA). 10-Acetyl-3,7-dihydroxyphenoxazine (Amplex Red) was synthesized according to [98 ]. All reagents and solvents used were analytical or HPLC grade.

Marcucci C., Rademacher M., Kamecki F., Pastore V., Bach H.G., Ricco R.A., Wagner M.L., Knez D., Gobec S., Colettis N, & Marder M. (2023). Biological Evaluation of Valeriana Extracts from Argentina with Potent Cholinesterase Inhibition for the Treatment of Neurodegenerative Disorders and Their Comorbidities—The Case of Valeriana carnosa Sm. (Caprifoliaceae) Studied in Mice. Pharmaceuticals, 16(1), 129.

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Hemoglobin Electrochemical Sensor using MWCNTs

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Multi-walled carbon nanotubes (MWCNTs, (30 ± 15) nm diameter, (1 -5) µm length and purity higher than 95%), were supplied from Nanolab (USA). Mesoporous silica particles MCM41 (MCM41, (102 ± 5) nm particle size, 2.1 nm pore size) were provided by Orion High Technologies (Spain). Human hemoglobin (Hb), hydroquinone and Nafion perfluorinated solution were supplied by Sigma. Hydrogen peroxide (30% v/v aqueous solution), NaH 2 PO 4 and Na 2 HPO 4 were purchased from Baker. Sodium nitrite and trichloroacetic acid (TCA) were obtained from Biopack. Other chemicals were of analytical grade and used without further purification.
A 0.050 M phosphate buffer solution pH 7.00 was employed as supporting electrolyte. Ultrapure water (ρ = 18.2 MΩ cm) from a Millipore-MilliQ system was used for preparing all aqueous solutions.

Eguílaz M., Villalonga R, & Rivas G. (2018). Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications. Biosensors & bioelectronics, 111.

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Soy Protein Concentrate Film Characterization

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Soy protein concentrate (SPC, Solcom S 110) with an average particle size of 100 mesh, and a proximate composition of 7% moisture, 69% protein, 1% fat, 3% fiber, 5% ash and about 15% nonstarch polysaccharides (mainly cellulose, non cellulose polymers and pectin polysaccharides), was provided by Cordis S.A. (Villa Luzuriaga, Buenos Aires, Argentina). Glycerol analytical grade (Gly, 98%) was purchased from Anedra (Buenos Aires, Argentina) and used as a plasticizer. TRIZMA/hydrochloric acid, glycine and Na 2 EDTA (Biopack, Buenos Aires, Argentina), Sodium dodecyl sulfate (SDS) and urea (Anedra, Buenos Aires, Argentina), and 2mercaptoethanol (Aldrich, St. Louis, USA) were used in solubility tests. Buffer solution pH 10 (Anedra, Buenos Aires, Argentina) was used to adjust the pH level of the film-forming solutions. Sodium azide (Na 3 N, Anedra, Buenos Aires, Argentina) was applied to prevent microbial growth during total soluble matter assays. Calcium chloride (CaCl 2 ; Aldrich, St. Louis, USA) was used as desiccant for water vapor transmission measurements. Copper sulfate (CuSO 4 ) and potassium and sodium double tartrate tetrahydrate (Anedra, Buenos Aires, Argentina) were used as received to prepare Biuret reagent. Trichloroacetic acid (TCA, Biopack, Buenos Aires, Argentina) was used as received.

Ciannamea E.M., Stefani P.M, & Ruseckaite R.A. (2015). Storage-induced changes in functional properties of glycerol plasticized – Soybean protein concentrate films produced by casting. Food Hydrocolloids., 45.

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Antioxidant Compounds Characterization

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Malondialdehyde bis(diethylacetal) or 1,1,3,3-tetraethoxypropane (TEP), hexanal, 1,1-diphenyl-2-picrilhidrazyl radical (DPPH • ), and chlorogenic and caffeic acids were purchased from Sigma (St. Louis, SA). Thiobarbituric acid (TBA) was obtained from Merck (Darmstast, Germany). Trichloroacetic acid (TCA) was purchased from Biopack (Buenos Aires, Argentina). Acetophenone was purchased from Anedra (Buenos Aires, Argentina). All aqueous solutions were prepared with Milli-Q water.

Yonny M.E., Medina A.V., Nazareno M.A, & Chaillou L.L. (2018). Enhancement in the oxidative stability of green peas by Ilex paraguariensis addition in a blanching process before their refrigerated and frozen storage. Lebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire, 91.

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