Neisseria gonorrhoeae (ATCC 43069) was obtained from ATCC (Manassas, VA). Bacterial dilutions (108 to 104 CFU/mL) were prepared from overnight cultures in distilled, autoclaved water and submitted to lysing by conventional heating and microwaves as described below. Microbial cells were lysed by heating 4 mL of bacterial suspensions (108 CFU/mL) in sterile scintillation vials fitted with a thermometer for temperature monitoring. Bacterial suspensions were heated to 40°, 50°, 60° and 70°C for 30, 60, or 90 seconds. These temperatures were selected to simulate temperatures reached during microwave irradiation. To determine culture survival, a 20 µL aliquot of each lysate was plated on chocolate agar plates and incubated overnight at 37°C.
Neisseria gonorrhoeae
Manufactured by American Type Culture Collection
Neisseria gonorrhoeae is a strain of bacteria maintained by the American Type Culture Collection (ATCC). It is a Gram-negative, aerobic diplococcus that is the causative agent of the sexually transmitted infection gonorrhea. The ATCC provides this strain for research and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Bacillus cereus, by American Type Culture Collection (1 mentions)
Rhodobacter sphaeroides, by American Type Culture Collection (1 mentions)
Bacillus subtillis, by American Type Culture Collection (1 mentions)
Escherichia coli, by American Type Culture Collection (1 mentions)
Staphylococcus aureus, by American Type Culture Collection (1 mentions)
2 protocols using neisseria gonorrhoeae
1
Thermal Lysis of Neisseria gonorrhoeae
2
Evaluating DNA Signature Specificity Across Species
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Genomic DNA from nonhuman organisms were tested in species specificity studies: two Old World primates (female baboon, Zyagen Laboratories, San Diego, CA; male rhesus monkey, BioChain 1 , Eureka, CA), nine non-primate mammals (male cat, male dog, mixed male and female ferret, female horse (Zyagen Laboratories, San Diego, CA)), and male cow, mixed male and female hamster, male mouse, male pig, male rat (BioChain 1 , Eureka, CA), one avian species (male domesticated chicken; Zyagen Laboratories, San Diego, CA), two fungal samples (Candida albicans, BioChain 1 , Eureka, CA; Saccharomyces cerevisiae, White Labs, San Diego, CA), and a pooled bacterial sample of six microorganisms that was treated as a single sample (Rhodobacter sphaeroides, Escherichia coli, Bacillus cereus; ATCC, Manassas, VA), Staphylococcus aureus, Neisseria gonorrhoeae, Bacillus subtillis (Cambridge University Hospital, Cambridge, United Kingdom). Baboon and monkey (1 ng) and all other nonhuman samples (10 ng) were amplified with DPMB and processed according to the ForenSeq TM DNA Signature Prep Guide (Illumina part #15049528) [77] .
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