Doxorubicin d 4000

Doxorubicin (D-4000) was purchased from LC Laboratories (Woburn, MA, USA). ε-poly-lysine (ε-PLL) was obtained from Zhengzhou Bainafo Bioengineering Co., Ltd (Zhengzhou, Henan, China). Fucoidan from Macrocystis pyrifera, gentian violet, phalloidin-FITC, dextran sulfate sodium salt and in situ cell death detection kit were purchased from Sigma (St. Louis, MO, USA). The XenoLight D-Luciferin-K⁺ salt bioluminescent substrate was purchased from PerkinElmer (Shelton, CT, USA). Carboxyl Modified Latex (CML) Beads (4% w/v, 0.1 μm), FluoSpheres™ Carboxylate-Modified Latex (CML) Latex Beads (2% w/v, 0.1 μm, 580/605), and P-selectin rabbit monoclonal antibody (3H20L10) were obtained from Thermo Scientific (Eugene, OR, USA). Recombinant human P-selectin/CD62P protein, recombinant human E-Selectin/CD62E protein, recombinant human L-Selectin/CD62L protein, and recombinant human TNF-alpha protein were purchased from R&D Systems (Minneapolis, MN, USA). Hydrogen peroxide solution (3%) and paraformaldehyde (4%) was purchased from Sigma (St. Louis, MO, USA). Dil and DiR dyes were obtained from Thermo Fisher Scientifics (Eugene, OR, USA)