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2 protocols using 1 heptadecanoyl 2 hydroxy sn glycero 3 phosphate

1

Lipid Extraction and Characterization Protocol

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Reagent-grade chemicals and HPLC-grade solvents were purchased from major commercial suppliers (Fisher Scientific, Hampton, NH, USA, and Sigma Aldrich, St. Louis, MO, USA). 1,2-Dipalmitoyl-sn-glycero-3-phospho-N-methylethanolamine (PME) was purchased from Santa Cruz Biotech (Santa Cruz, CA, USA), and 1-(10Z-heptadecenoyl)-2-hydroxy-sn-glycero-3-phosphocholine (LPC(17:1)), 1-(10Z-heptadecenoyl)-sn-glycero-3-phosphoethanolamine (LPE(17:1)), 1-(10Z-heptadecenoyl)-2-hydroxy-sn-glycero-3-phospho-(1’-myo-inositol) (LPI(17:1)), and 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphate (LPA(17:0)) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Milli-Q water was used throughout.
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2

Lipidomic Internal Standards Protocol

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1-Heptadecanoyl-2-hydroxy-sn-glycero-3-phosphate (sodium salt) (LPA 17:0), 1,2-dimyristoyl-sn-glycero-3-phosphate (sodium salt) (PA 14:0/14:0), N-lauroyl-d-erythrosphingosylphosphorylcholine (SM d18:1/12:0), 1-myristoyl-2-hydroxy-sn-glycero-3-phospho-(1 -rac-glycerol) (sodium salt) (LPG 14:0), 1,2-diheptadecanoyl-sn-glycero-3-phospho-(1 -rac-glycerol) (sodium salt) (PG 17:0/17:0), 1-nonadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (LPC 19:0), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (PC 14:0/14:0), 1,2-dimyristoyl-sn-glycero-3-phospho-l-serine (sodium salt) (PS 14:0/14:0), 1-myristoyl-2-hydroxysn-glycero-3-phosphoethanolamine (LPE 14:0), and 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (PE 14:0/14:0) were purchased from Avanti Polar Lipids (Alabaster, USA) and used as internal standards (IS). Water, methanol, acetonitrile, ammonium formate (all LC-MS grade), n-hexane, and chloroform (HPLC grade) were purchased from VWR (Radnor, USA). LC-MS-grade 2-propanol was purchased from Fluka (Buchs, Switzerland) and acetone (GC-MS grade) was from MERCK (Darmstadt, Germany).
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