Rabbit polyclonal antibody against h3k27me3

Manufactured by Merck Group

Sourced in United States

Rabbit polyclonal antibody against H3K27me3 is a laboratory reagent used for the detection and analysis of the trimethylation of lysine 27 on histone H3 protein. This modification is associated with transcriptional repression and the maintenance of heterochromatin. The antibody can be used in various applications, such as Western blotting, immunoprecipitation, and chromatin immunoprecipitation (ChIP).

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Lab products found in correlation

Biotin conjugated anti rabbit antibody, by Thermo Fisher Scientific (2 mentions) Secondary antibody anti mouse biotin conjugated with fluorescein, by Jackson ImmunoResearch (2 mentions) Xcp x orange, by MetaSystems (1 mentions) Rabbit anti dnp antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Anti rabbit secondary antibody, by Abcam (1 mentions)

Close spelling variants of this product

H3K27me3 antibody H3K27me3

2 protocols using rabbit polyclonal antibody against h3k27me3

Multi-Modal Fluorescent Imaging Protocols

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For DNA FISH, secondary antibody anti-sheep digoxigenin conjugated with fluorescein (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) was diluted 1:250. For RNA/DNA FISH with FOXP3-DNP probes, rabbit anti-DNP antibody (Thermo Fisher Scientific) was diluted 1:1000. Next, secondary antibody sheep anti-digoxigenin conjugated with fluorescein (Jackson ImmunoResearch Laboratories) was diluted 1:250 and goat anti-rabbit alexa fluor 594 (Thermo Fisher Scientific) was diluted 1:1000. For immuno-DNA FISH, rabbit polyclonal antibody against H3K27me3 (Millipore Sigma) was diluted 1:50 followed by biotin conjugated anti-rabbit antibody (Thermo Fisher Scientific) was diluted 1:250 and secondary antibody anti-mouse biotin conjugated with fluorescein (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) was diluted 1:250. For immuno-DNA FISH containing FOXP3-DNP probes, rabbit anti-DNP antibody (Thermo Fisher Scientific) was diluted 1:1000 and secondary goat anti-rabbit alexa fluor 594 (Thermo Fisher Scientific) was diluted 1:1000. A total of 8 µl of human chromosome X paint (XCP X Orange, MetaSystems) was hybridized per 22 × 22 mm square coverslip for fibroblasts and 6 µl was hybridized for the differentiation assay and lymphocytes plated on 12 mm round coverslips.

Laskowski A.I., Neems D.S., Laster K., Strojny-Okyere C., Rice E.L., Konieczna I.M., Voss J.H., Mathew J.M., Leventhal J.R., Ramsey-Goldman R., Smith E.D, & Kosak S.T. (2019). Varying levels of X chromosome coalescence in female somatic cells alters the balance of X-linked dosage compensation and is implicated in female-dominant systemic lupus erythematosus. Scientific Reports, 9, 8011.

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Histone Modification Analysis in Cells

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Rabbit polyclonal antibody against H3K27me3 (Millipore Sigma, St Louis, Missouri) was diluted 1:1000 or 1:50 for Western blot or immuno-DNA fluorescence in situ hybridization (FISH), respectively. Rabbit polyclonal antibody against acetyl-histone H3 (Millipore Sigma) was diluted 1:2000 and rabbit polyclonal antibody against histone H3 (Abcam, Cambridge, United Kingdom) was diluted 1:4000 for Western blot. Antirabbit secondary antibody (Abcam) conjugated with horseradish peroxidase was diluted 1:5000. For immuno-DNA FISH, biotin-conjugated antirabbit antibody (Thermo Fisher Scientific) was diluted 1:250 and secondary antibody antimouse biotin conjugated with fluorescein (Jackson ImmunoResearch Laboratories, West Grove, Pennsylvania) was diluted 1:250. A total of 8 μL of human chromosome X paint (MetaSystems, Heidelberg, Germany) was hybridized per 22 mm × 22 mm coverslip.

Laskowski A.I., Fanslow D.A., Smith E.D, & Kosak S.T. (2018). Clinical Epigenetic Therapies Disrupt Sex Chromosome Dosage Compensation in Human Female Cells. Gender and the genome, 2(1), 2-7.

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