1 2 dipalmitoyl sn glycero 3 phosphatidylcholine

Manufactured by Avanti Polar Lipids

Sourced in United States

1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine is a common phospholipid compound used in various laboratory applications. It is a synthetic analog of naturally occurring phosphatidylcholine, which is a major component of cell membranes. The compound serves as a model system for studying membrane structure and function.

Automatically generated - may contain errors

Lab products found in correlation

Chloroform, by Merck Group (4 mentions) Methanol, by Merck Group (3 mentions) Melanin, by Merck Group (1 mentions) Calcein acetoxymethyl ester calcein am, by Santa Cruz Biotechnology (1 mentions) 1 1 dioctadecyl 3 3 3 3 tetramethylindotricarbocyanine iodide dir, by Beyotime (1 mentions) Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions) Propidium iodide, by Merck Group (1 mentions) 2 4 amidinophenyl 6 indolecarbamidine dihydrochloride dapi, by Beyotime (1 mentions) 1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate dii, by Beyotime (1 mentions) Milli q water, by Merck Group (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphatidylcholine, by Avanti Polar Lipids (1 mentions) Oleic acid, by Cayman Chemical (1 mentions) Eicosapentaenoic acid, by Cayman Chemical (1 mentions) Docosahexaenoic acid, by Cayman Chemical (1 mentions) Diphenylhexatriene dph, by Merck Group (1 mentions) Egg phosphatidylcholine, by Avanti Polar Lipids (1 mentions) Egg sphingomyelin, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 docosahexaenoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 2 dimyristoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (1 mentions) Etoposide, by Bio-Techne (1 mentions)

Close spelling variants of this product

Phosphatidylcholine

8 protocols using 1 2 dipalmitoyl sn glycero 3 phosphatidylcholine

Lipid-based Nanoparticle Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Melanin and propidium iodide (PI) were purchased from Sigma Aldrich (St. Louis, MO, USA). 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (DSPE-mPEG2000) and cholesterol were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Calcein acetoxymethyl ester (Calcein-AM) was purchased from Santa Cruz Biotechnology (TX, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Japan). 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI), 2-(4-Amidinophenyl)-6-indolecarbamidinedihydrochloride (DAPI) and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR) were obtained from Beyotime Technology. Deionized water was obtained from a Millipore water purification system. All reagents were used without further purification.

Zhang L., Sheng D., Wang D., Yao Y., Yang K., Wang Z., Deng L, & Chen Y. (2018). Bioinspired Multifunctional Melanin-Based Nanoliposome for Photoacoustic/Magnetic Resonance Imaging-Guided Efficient Photothermal Ablation of Cancer. Theranostics, 8(6), 1591-1606.

+ Open protocol

+ Expand

Lipid Bilayer Composition Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Figure 1 depicts
the compounds used in this work. 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC), 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), cholesterol
(ovine wool) (chol), 7β-hydroxycholesterol (7β-OH-chol),
27-hydroxycholesterol (27-OH-chol), 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (16:0-NBD-PE), 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (18:1-NBD-PE), and 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine-N-(cap biotinyl) (biotinylated DOPE) were obtained from Avanti Polar
Lipids, Inc. (Alabaster, AL). 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic
acid (HEPES), sodium chloride, ethylenedinitrilotetraacetic
acid (EDTA), tris(hydroxymethyl)aminomethane (Tris), and
sodium dithionite were ordered from Sigma-Aldrich (St. Louis, MO).
Buffers were prepared using Milli-Q water (Millipore, USA). Merck
(Darmstadt, Germany) supplied organic solvents of spectroscopic grade.
All chemicals were used without further purification.

Olżyńska A., Kulig W., Mikkolainen H., Czerniak T., Jurkiewicz P., Cwiklik L., Rog T., Hof M., Jungwirth P, & Vattulainen I. (2020). Tail-Oxidized Cholesterol Enhances Membrane Permeability for Small Solutes. Langmuir, 36(35), 10438-10447.

+ Open protocol

+ Expand

Lipid Composition for Liposome Formulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phospholipids such as 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC, purity ≥
99.0%), 1,2-dilauroyl-sn-glycero-3-phosphate (sodium
salt, PA, purity ≥ 99.0%), and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene
glycol)-5000] (ammonium salt, PEG5000-PE, purity ≥ 99.0%) were
obtained from Avanti Polar lipids Inc., Alabaster, AL. Cholesterol
(purity ≥ 99.0%) was purchased from Sigma-Aldrich Inc., St.
Louis, MO. Fluorescently labeled phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphatidylethanolamine by Atto 488
(Atto 488 DMPE, purity ≥ 80.0%) was also obtained from Sigma-Aldrich
Inc., St. Louis, MO. The solvents for liposome preparation, chloroform,
and methanol were obtained from Penta s.r.o. and Sigma-Aldrich Inc.,
St. Louis, MO, respectively. Pure water was obtained from ELGA LabWater
Ltd., Lane End, UK.

Mišík O., Kejíková J., Cejpek O., Malý M., Jugl A., Bělka M., Mravec F, & Lízal F. (2024). Nebulization and In Vitro Upper Airway Deposition of Liposomal Carrier Systems. Molecular Pharmaceutics, 21(4), 1848-1860.

+ Open protocol

+ Expand

Lipid Membrane Composition Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chloroform, dichloromethane and methanol, HPLC-grade solvents, were obtained from Merck (Darmstadt, Germany). Egg phosphatidylcholine (PC), egg sphingomyelin (SM), 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) and 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (PDPC) were obtained from Avanti Polar Lipids (Birmingham, AL, USA). Diphenyl hexatriene (DPH) was supplied by Sigma-Aldrich (Madrid, Spain). Docosahexaenoic acid, eicosapentaenoic acid and oleic acid were provided by Cayman Chemical (Ann Arbor, MI).

De Santis A., Varela Y., Sot J., D’Errico G., Goñi F.M, & Alonso A. (2018). Omega-3 polyunsaturated fatty acids do not fluidify bilayers in the liquid-crystalline state. Scientific Reports, 8, 16240.

+ Open protocol

+ Expand

Acetylcholinesterase Assay with Lipids

Check if the same lab product or an alternative is used in the 5 most similar protocols

1,2-dimyristoyl-sn-glycero-3-phospho-L-serine (DMPS), 1,2-dimyristoyl-sn-glycero-3-phosphatidic acid (DMPA), 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) were obtained from Avanti Polar Lipids. Acetylcholinesterase from E. electricus (Type V-S and Type VI-S, specific activity ≥1 Unit/

μ

g) was purchased from Sigma Aldrich. All other reagents were obtained from Sigma-Aldrich and were of analytical purity (≥99%).

Fillafer C., Koll Y.S, & Schneider M.F. (2021). Lipid Membrane State Change by Catalytic Protonation and the Implications for Synaptic Transmission. Membranes, 12(1), 5.

+ Open protocol

+ Expand

Phospholipid Profiling of Camel Milk

Check if the same lab product or an alternative is used in the 5 most similar protocols

1,2-Dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), synthetic soya phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), and lysophosphatidylcholine (LPC) standards were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Silica gel-60 and precoated thin layer chromatography (TLC) plates, triethyamine, acetonitrile, methanol, chloroform, and phosphoric acid of HPLC grade were purchased from Merck (Germany). Etoposide was obtained from Tocris Bioscience (UK). Sephadex G-75 was obtained from Fisher Scientific (Loughborough, UK). Camel milk was collected from a six-year-old red camel two months postdelivery lactation period from Aljarbou farm in Qassim, Saudi Arabia.

Maswadeh H.M., Aljarbou A.N., Alorainy M.S., Alsharidah M.S, & Khan M.A. (2015). Etoposide Incorporated into Camel Milk Phospholipids Liposomes Shows Increased Activity against Fibrosarcoma in a Mouse Model. BioMed Research International, 2015, 743051.

+ Open protocol

+ Expand

Doxorubicin-Loaded Lipid Nanoparticle Formulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC),
1-stearoyl-2-hydroxy-sn-glycero-3-phosphatidylcholine (MSPC), and
1,2-distearoyl-sn-glycero-3-phosphatidyl ethanol amine-N-[methoxy(polyethylene
glycol)-2000] – ammonium salt (DSPE-PEG 2000) were obtained from Avanti Polar
Lipids (Alabaster, AL, USA) and used without further purification. Doxorubicin-HCl,
citric acid, Sephadex G-50, HEPES sodium salt, sodium carbonate anhydrous, and other
chemicals (chloroform, methanol and hydrochloric acid) were obtained from Sigma-Aldrich
(St Louis, MO). All chemicals involved were standard reagent grade and used without
further purification.

Viglianti B.L., Dewhirst M.W., Boruta R.J., Park J.Y., Landon C., Fontanella A.N., Guo J., Manzoor A., Hofmann C.L, & Palmer G.M. (2014). Systemic anti-tumour effects of local thermally sensitive liposome therapy. International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, 30(6), 385-392.

+ Open protocol

+ Expand

Biophysical Characterization of Antimicrobial Peptide LL-III

Check if the same lab product or an alternative is used in the 5 most similar protocols

The peptide lasioglossin-III (LL-III) with the sequence NH 2 -VNWKKILGKIIKVVK-CONH 2 was purchased from Primm Srl (Milano, Italy) with a purity of 495%. The lipids cholesterol (Chol), 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), 1-palmitoyl-2oleoyl-sn-glycero-3-phosphatidylserine (POPS) and 1-palmitoyl-2-oleoyl-glycero-3-phosphatidylcholine (POPC) as well as spin-labeled phosphatidylcholines used as EPR probes (1-palmitoyl-2-stearoyl-(n-doxyl)-sn-glycero-3-phosphatidylcholine, nPC-SL, n = 5, 7, 10, and 14) were provided by Avanti Polar Lipids (Alabaster, United States). The fluorescent probes, 1,6-diphenylhexatriene (DPH) and 6-dodecanoyl-2-dimethylaminonaphthalene (Laurdan), chloroform, methanol, dimethylformamide (DMF), cacodylic acid, calcium chloride (CaCl 2 ), sodium chloride (NaCl), sodium hydroxide (NaOH) and carboxyfluorescein (CF) were acquired from Merck (Darmstadt, Germany). Sodium cacodylate buffer (10 mM) was prepared by solubilizing cacodylic acid in deionized water and adjusting the pH to 6.5 or 7.0 by adding an appropriate amount of NaOH. The pH values of 6.5 and 7.0 were selected to mimic tumor and healthy cell extracellular matrices, respectively. 17

Campanile M., Oliva R., D'Errico G., Del Vecchio P, & Petraccone L. (2023). The anticancer peptide LL-III alters the physico-chemical properties of a model tumor membrane promoting lipid bilayer permeabilization. Physical chemistry chemical physics : PCCP, 25(5).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!