0.22 μm syringe filter

Manufactured by Pall Corporation

Sourced in United States

The 0.22 μm syringe filter is a laboratory filtration device designed to remove particulates and microorganisms from liquid samples. It features a 0.22 μm pore size membrane that effectively retains bacteria and other small particles, ensuring the filtrate is free from contaminants.

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Lab products found in correlation

Ribogreen reagent, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Mabselect sure, by GE Healthcare (1 mentions) Slide a lyzer dialysis flasks, by Thermo Fisher Scientific (1 mentions) Octet red96, by Pall Corporation (1 mentions) Compstatin, by Bio-Techne (1 mentions) Monensin, by Merck Group (1 mentions) 1200 series, by Agilent Technologies (1 mentions)

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Syringe filters (10 citations)

6 protocols using 0.22 μm syringe filter

Copolymer-Based RNA Nanoparticle Formulation

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Lyophilized copolymers
were dissolved into ethanol at 50 mg/mL and stored at 4 °C. This
stock was further diluted to 3.33 mg/mL in citric acid buffer (pH
4, 100 mM) and rapidly mixed with 3pRNA or OH-RNA at charge ratios
(N:P) between 20:1 and 1:1. After incubating at room temperature for
30 min, 1.24× volume phosphate buffer (pH 8, 100 mM) was added
and mixed rapidly to form nanoparticles (NPs). After 15 min, the solution
was further diluted into 1× PBS (pH 7.4, Gibco) before use. The
second block DMAEMA content is estimated to have 50% protonation for
the purposes of determining N:P ratios. A charge ratio of 20:1 was
selected for all in vitro cell culture studies. The
same approach was used to prepare formulations for in vivo studies, except that they were suspended at 10 mg/mL in citric acid
buffer instead of 3.33 mg/mL, phosphate buffer was added at a ratio
of 1:1.26, a charge ratio of 15:1 was used, and formulations were
filtered using a 0.22 μm syringe filter (Pall corporation).
3pRNA complexation efficiency was determined by quantifying free 3pRNA
after NP/3pRNA formulation with the fluorescence-based RiboGreen reagent
(Invitrogen) according to the manufacturer’s instructions.

Jacobson M.E., Becker K.W., Palmer C.R., Pastora L.E., Fletcher R.B., Collins K.A., Fedorova O., Duvall C.L., Pyle A.M, & Wilson J.T. (2020). Structural Optimization of Polymeric Carriers to Enhance the Immunostimulatory Activity of Molecularly Defined RIG-I Agonists. ACS Central Science, 6(11), 2008-2022.

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Purification of Bispecific Antibodies

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The concentration of parental antibodies in culture media was quantitated via Biolayer Light Interferometry (BLI) using ForteBio anti-human Fab-CH1 second Generation (FAB2G) biosensors on an Octet RED96 instrument (ForteBio; Pall Life Sciences, Fremont, CA, USA). Then, cultures from F405L and K409R RF constructs were mixed at a ratio of 1:1.2 and then reduced using 75 mM of β-mercaptoethanol (βME) for 5 h at 31 °C before dialysis in phosphate-buffered saline(PBS) at 4 °C overnight with Slide-A-Lyzer™ Dialysis Flasks, 10K molecular weight cut-off (MWCO), 250 mL (Thermo Scientific™, Waltham, MA, USA). Antibodies were then affinity purified using 5mL MabSelect™ SuRe™ (General Electric Healthcare, Chicago, IL, USA) using the manufacturer’s specifications. After elution, bsAbs were filtered using a 0.22 μm syringe filter (Pall Corporation, New York, NY, USA).

Steinhardt J., Wu Y., Fleming R., Ruddle B.T., Patel P., Wu H., Gao C, & Dimasi N. (2019). Fab-Arm Exchange Combined with Selective Protein A Purification Results in a Platform for Rapid Preparation of Monovalent Bispecific Antibodies Directly from Culture Media. Pharmaceutics, 12(1), 3.

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Conditioned Media Characterization and Modification

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MSC- or MDF-derived CM was harvested 3 days post incubation. For some experiments, CM was subjected to some modifications; CM was filtered using a 0.22-μm syringe filter (Pall Corporation, Port Washington, NY, USA); filtered CM was heat-inactivated at 100 °C for 5 min; CM was treated with different doses (15–60 μM) of compstatin (TOCRIS, Bristol, UK), which is a C3 inhibitor; CM was separated using filter units with a molecular weight cutoff size of 50 or 100 kDa (Millipore, Billerica, MA, USA). For FPLC separation and western blot analysis, 200 ml of filtered CM, which was harvested 3 days post incubation in the absence of serum, were concentrated (200 × ) using filter units with a molecular weight cutoff size of 50 kDa. For ER/Golgi block, cells were incubated for 24 h with 2 μM of monensin (Sigma, St. Louis, MO, USA), which blocks the protein transport from the endoplasmic reticulum to the Golgi apparatus. Then, CM was harvested and filtered by using a 0.22-μm syringe filter.

Lee D.S., Yi T.G., Lee H.J., Kim S.N., Park S., Jeon M.S, & Song S.U. (2014). Mesenchymal stem cells infected with Mycoplasma arginini secrete complement C3 to regulate immunoglobulin production in b lymphocytes. Cell Death & Disease, 5(4), e1192-.

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Quantifying Brain Dopamine Levels

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Peripubertal and young adult mice were euthanized and their brain tissue was dissected and snap-frozen using liquid nitrogen. Samples were sonicated in 200μL of 0.1N perchloric acid and, after centrifugation at 14,000xg for 15 minutes, the supernatant was filtered through a 0.22μm syringe filter (PALL Corp). Tissue levels of dopamine were measured by HPLC analysis coupled with electrochemical detection as described^{16 (link)}. Fast isocratic separation was obtained using a reverse phase TSK gel Super-ODS C-18 column (4.6mm x 100mm, 2μm particle size; Tosoh Bioscience) with the following mobile phase: 30mM sodium citrate, 13.7mM sodium monobasic phosphate, 0.025mM Na₂EDTA, 0.75mM sodium octanesulphonate; pH 3.15-3.2, containing 0.6 % tetrahydrofuran, 0.1% diethylamine, and 6.5% acetonitrile.

Stutz B., Waterson M.J., Šestan-Peša M., Dietrich M.O., Škarica M., Sestan N., Racz B., Magyar A., Sotonyi P., Liu Z.W., Gao X.B., Matyas F., Stoiljkovic M, & Horvath T.L. (2022). AgRP neurons control structure and function of the medial prefrontal cortex. Molecular psychiatry, 27(10), 3951-3960.

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Bacterial Culture Broth Preparation

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To obtain bacterial culture broths, different strains of Photorhabdus or Xenorhabdus bacteria were cultured in 100 mL of LB for 48 h at 28°C until OD600 reached 2.0. The culture medium was then centrifuged at 8000 r/min for 20 min. The supernatant was collected and filtered through 0.22 μm syringe filter (Pall Corp., Ann Arbor, MI, United States) to remove bacterial cells.

Sajjadian S.M, & Kim Y. (2020). Dual Oxidase-Derived Reactive Oxygen Species Against Bacillus thuringiensis and Its Suppression by Eicosanoid Biosynthesis Inhibitors. Frontiers in Microbiology, 11, 528.

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Radiolabeling and Characterization of 18F-FAPI-04

Cited 1 time

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¹⁸F-FAPI-04 was prepared as described previously (17 (link),18 (link)). The NOTA-FAPI-04 precursor was purchased from Beijing PET Technology Co. Ltd. ¹⁸F was produced from a medical cyclotron (Siemens Medical Solutions). The synthesis of ¹⁸F-FAPI-04 was performed in an AllInOne synthesis module (Trasis). The final product was reconstituted in saline and passed through a 0.22-μm syringe filter (Pall Corp.). The radiochemical purity of ¹⁸F-FAPI-04 was analyzed by radio–high-performance liquid chromatography (1200 series; Agilent) and was more than 95%. ¹⁸F-FDG was synthesized automatically and routinely in a ¹⁸F-FDG synthesizer module (FDG4 Explora; Siemens) and was purified to radiochemical purity of more than 95% before clinical use.

Li X., Lu N., Lin L., Chen Y., Yang S., Wang H., Liu X., Wu C., Xue X., Su X., Bai X, & Liang T. (2024). 18F-FAPI-04 Outperforms 18F-FDG PET/CT in Clinical Assessments of Patients with Pancreatic Adenocarcinoma. Journal of Nuclear Medicine, 65(2), 206-212.

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