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Tbars assay kit

Manufactured by Cambridge Bioscience
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Sourced in United Kingdom

The TBARS Assay kit is a colorimetric assay designed to measure the levels of thiobarbituric acid-reactive substances (TBARS), which are a byproduct of lipid peroxidation. The kit provides a simple and convenient method for the quantification of TBARS in biological samples, such as tissues, cells, and body fluids.

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Lab products found in correlation

Protein carbonyl colorimetric assay kit, by Cambridge Bioscience (2 mentions) Anti tubulin, by Cell Signaling Technology (1 mentions) Anti bax, by Cell Signaling Technology (1 mentions) Anti fas, by Cell Signaling Technology (1 mentions) Anti apaf 1, by Cell Signaling Technology (1 mentions) Anti caspases 8 and 9, by Cell Signaling Technology (1 mentions) Anti fadd, by Cell Signaling Technology (1 mentions) Anti bak, by Cell Signaling Technology (1 mentions) Anti bid, by Cell Signaling Technology (1 mentions)

4 protocols using tbars assay kit

1

Lipid Peroxidation Assay in A375 Cells

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Standard conditions were used as previously described [18] . All antibodies (e.g., anti-Caspases-8 and -9, anti-Apaf-1, anti-BID, anti-FADD, anti-FAS, anti-BAX, anti-BAK and anti-Tubulin) were purchased from Cell Signaling Technology (Danvers, MA, USA) and utilized according to the manufacturer's protocol.
2.9. Determination of lipid peroxidation content A375 cells were plated in 100 mm dishes, cultured overnight and next day were treated with L-SK-4 (100 µM). After trypsinization, pellets were collected, re-suspended and sonicated before the TBARS Assay kit (Cambridge Bioscience Ltd, Cambridge, UK) was utilized for the determination of malondialdehyde (MDA) content according to the manufacture's protocol.
Kyriakou S., Cheung W.K., Mantso T., Mitsiogianni M., Anestopoulos I., Veuger S., Trafalis D., Franco R., Pappa A., Tétard D., & Panayiotidis M.I. (2021). A novel methylated analogue of L-Mimosine exerts its therapeutic potency through ROS production and ceramide-induced apoptosis in malignant melanoma.
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2

Measuring Oxidative Stress in A375 Cells

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A375 cells were plated in 100 mm dishes, cultured overnight and next day were treated with L-SK-4 (100 μM). After trypsinization, pellets were collected, re-suspended and sonicated before the TBARS Assay kit (Cambridge Bioscience Ltd., Cambridge, UK) was utilized for the determination of malondialdehyde (MDA) content according to the manufacture’s protocol.
Kyriakou S., Cheung W., Mantso T., Mitsiogianni M., Anestopoulos I., Veuger S., Trafalis D.T., Franco R., Pappa A., Tetard D, & Panayiotidis M.I. (2021). A novel methylated analogue of L-Mimosine exerts its therapeutic potency through ROS production and ceramide-induced apoptosis in malignant melanoma. Investigational New Drugs, 39(4), 971-986.
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3

Oxidative Stress Biomarkers in A375 Cells

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A375 cells were plated in 100 mm dishes (1.4 × 106 and 0.7 × 106 for 24 and 48 h, respectively) and cultured overnight. On the next day, cells were treated with either N-acetyl cysteine (NAC) (2.5 mM), tert-butyl hydroperoxide (TBH) (200 μΜ) or SF3 (0.05 mg/mL) for 24 and 48 h, respectively. After trypsinization, pellets were collected, re-suspended in PBS and sonicated. For the determination of the lipid peroxidation content, the whole suspension was further diluted with 4 mL of 4% v/v acetic acid solution containing 8% TBA and 0.1% SDS. The final mixture was heated at 95 °C for 1 h and centrifuged at 3000 rpm for 2 min. The TBARS Assay kit (Cambridge Bioscience Ltd., Cambridge, UK) was utilized for the determination of malondialdehyde (MDA) content according to the manufacture’s protocol. For the determination of protein carbonyl content, cells were trypsinized and pellets were collected, re-suspended in PBS (supplemented with 1 mM EDTA) and sonicated. The Protein Carbonyl Colorimetric Assay Kit (Cambridge Bioscience Ltd., Cambridge, UK) was utilized according to the manufacture’s protocol.
Kyriakou S., Tragkola V., Plioukas M., Anestopoulos I., Chatzopoulou P.S., Sarrou E., Trafalis D.T., Deligiorgi M.V., Franco R., Pappa A, & Panayiotidis M.I. (2021). Chemical and Biological Characterization of the Anticancer Potency of Salvia fruticosa in a Model of Human Malignant Melanoma. Plants, 10(11), 2472.
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4

Oxidative Stress Evaluation in A375 Cells

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A375 cells were plated in 100 mm dishes (1.4 × 106, 0.7 × 106 and 0.4 × 106 per dish for 24, 48 and 72 h, respectively) and cultured overnight. The next day, cells were treated with either N-acetyl cysteine (NAC) (2.5 mM), tert-butyl hydroperoxide (TBH) (200 μΜ), synthetic PEITC (28, 12 and 7 μM) or edible (16, 10 and 9 μM) or non-edible (32, 18 and 13 μM) watercress samples for 24, 48 and 72 h, respectively. After trypsinization, pellets were collected, re-suspended in PBS and sonicated. For the determination of lipid peroxidation content, the whole suspension was further diluted with 4 mL of 4% v/v acetic acid solution containing 8% TBA and 0.1% SDS. The final mixture was heated at 95 °C for 1 h and centrifuged at 3000 rpm for 2 min. The TBARS Assay kit (Cambridge Bioscience Ltd., Cambridge, UK) was utilized for the determination of malondialdehyde (MDA) content according to the manufacture’s protocol. For the determination of protein carbonyl content, cells were trypsinized and pellets were collected, re-suspended in PBS (supplemented with 1 mM EDTA) and sonicated. The Protein Carbonyl Colorimetric Assay Kit (Cambridge Bioscience Ltd., UK) was used according to the manufacture’s protocol.
Kyriakou S., Tragkola V., Alghol H., Anestopoulos I., Amery T., Stewart K., Winyard P.G., Trafalis D.T., Franco R., Pappa A, & Panayiotidis M.I. (2022). Evaluation of Bioactive Properties of Lipophilic Fractions of Edible and Non-Edible Parts of Nasturtium officinale (Watercress) in a Model of Human Malignant Melanoma Cells. Pharmaceuticals, 15(2), 141.
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