The collected soft ticks were morphologically identified to genus level using a standard identification key [37 ]. Representative specimens were photographed at 50-200× magnification using a Keyence VHX 900F microscope (Keyence, Itasca, IL).
Vhx 900f microscope
Manufactured by Keyence
Sourced in United States
The VHX 900F microscope is a digital microscope designed for high-resolution imaging and measurement. It features a high-definition CMOS camera and advanced optics for clear and detailed observations. The VHX 900F is capable of capturing high-quality images and performing accurate measurements across a wide range of magnification levels.
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Lab products found in correlation
3 protocols using vhx 900f microscope
1
Tick Genus Identification Protocol
2
Tick Identification and Molecular Analysis
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Ticks were examined with a Keyence VHX-900F microscope (Itasca, IL, USA) and identified to species level using morphological keys (Filippova 1977 ; Pérez-Eid 2007 ; Hornok et al. 2014 (link)). In cases of questionable specimens, those were objected to molecular analysis. DNA was extracted using the QIAamp mini DNA extraction kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions. The 16S rRNA gene was amplified by PCR and Sanger sequenced according to Halos et al. (2004 (link)), using the tick-specific primer pair TQ16S + 1F (5’-CTGCTCAATGATTTTTTAAATTGCTGTGG-3’) and TQ16S-2R (5’-ACGCTGTTATCCCTAGAG-3’) of Black and Piesman (1994 (link)). Sequences were edited and primers trimmed using the Geneious Prime 2022.2.1 software (https://www.geneious.com ). A BLASTN search (Zhang et al. 2000 (link)) in GenBank was performed to molecularly annotate the individual 16S rDNA sequences. 16S rDNA sequence data can be found in GenBank and the supplementary material.
3
Multimodal Microscopic Imaging of Specimens
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For photography, A Keyence VHX-900F Microscope (Keyence Itasca, IL, USA) was used at 50× to 200× magnification. Helicon Focus 6.7.1 was used to combine image stacks. For computed tomography (micro-CT), specimens were mounted on a specimen holder and scanned using a Zeiss Xradia MicroXCT-400 (Carl Zeiss X-Ray Microscopy, Pleasanton, CA, USA) at 40 kVp tube voltage and 114 μA current using the 4X detector assembly. Depending on specimen size, isotropic voxel size in reconstructed volumes ranged between 1.40 μm and 2.90 μm. Reconstructed image volumes were processed and visualized by volume rendering and manual segmentation-based surface rendering using the 3D software package Amira 2022.1 (FEI SAS, a part of. Thermo Fisher Scientific). Drawings were prepared with a camera lucida attachment on a Leica M205C stereomicroscope (Leica Microsystems, Wetzlar, Germany), again using a combination of incident and transmitted light where appropriate. Artist's impressions were made from a combination of photographs and micro-CT scans.
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